ROLE OF VISION IN ETIOLOGY OF AXIAL MYOPIA

视力在轴性近视病因学中的作用

• 批准号: 3483962
• 负责人: JOSHUA WALLMAN
• 金额: $ 25.06万
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3483962
• 关键词: Prosimii; cell growth regulation; cellular pathology; chickens; chondrocytes; choroid uvea; dopamine; eye accommodation; eye refraction disorder; glycoprotein biosynthesis; insulinlike growth factor; morphology; myopia; pathologic process; phenotype; proteoglycan; radiotracer; retina; retinal pigment epithelium; retinoate; sclera; tissue /cell culture; visual deprivation

DESCRIPTION (Investigator's Abstract): This project seeks to contribute to the understanding of the etiology of myopia, a leading cause of blindness, by studying the severe myopia produced by deprivation of form vision in chickens. This animal model has three important features: the visual deprivation of one part of the retina produces myopia of only that part of the eye, and the scleral growth can be modulated up or down as necessary to compensate for myopia or hyperopia, suggesting that the eye can sense its refractive error and compensate for it. This has important implications in understanding how the eye grows and how it can go awry in clinical myopia.Three sets of experiments are proposed: (i) To study the cellular basis of myopia and emmetropization, the bidirectional modulation of scleral growth will be investigated in an in vitro system as assessed by incorporation of radiolabeled sulfur into in organ cultures and in dissociated cell cultures. The increase in proteoglycan synthesis in myopic scleras and cells of myopic eyes, and the decrease in those of recovering eyes will be studied to determine whether the modulation is achieved via a growth factor continuously or transiently released. Conditioned medium experiments will investigate the experiments will investigate the roles of he retinal pigment epithelium, choroid and retina as sources of relevant growth factors. Finally, different known growth factors will be added to the cultures to determine their effect on scleral growth in vitro. (ii) By in vivo studies, evidence will be sought that the recognized role of dopamine in the amelioration in form deprivation myopia represents a specific inhibition of the deprivation effect by using agonists on partially occluded eyes.In addition, scleras of tree shrews, which provide a mammalian model for myopia, will be studied to determine whether the myopia in these animals is also largely due to enhanced growth of the posterior sclera. (iii) The nature of the emmetropization process will be explored with experiments directed at determining the roles of the choroid and sclera in compensating for myopia or hyperopia.

描述（调查员的摘要）：该项目旨在贡献 理解近视的病因，这是 失明，通过研究剥夺形式产生的严重近视 鸡的视力。该动物模型具有三个重要特征： 视觉剥夺视网膜的一部分只会产生近视 一部分眼睛，并且可以根据 补偿近视或远视所必需的，表明眼睛 可以感觉到它的折射率并补偿了它。 这很重要 了解眼睛的生长以及如何出现的含义 临床近视。提出了三套实验：（i）研究 双向调制的近视和弹性化的细胞基础 巩膜生长将在体外系统中进行研究，如评估 通过将放射性标记的硫掺入器官培养物和 解离的细胞培养物。蛋白聚糖合成中的增加 近视巩膜和近视眼细胞，以及 将研究恢复的眼睛以确定调制是否是 通过生长因子连续或瞬时释放实现。 条件培养基实验将研究实验 研究视网膜色素上皮，脉络膜和视网膜的作用 作为相关增长因素的来源。最后，已知不同的增长 因素将被添加到培养物中，以确定其对硬化的影响 体外生长。 （ii）通过体内研究，将寻求证据表明 多巴胺在改善形式剥夺中的公认作用 近视是对剥夺效应的特定抑制 激动者部分被遮住的眼睛。加上树木的巩膜， 将研究为近视提供哺乳动物模型，以确定 这些动物中的近视是否也主要是由于增长增长 后巩膜。 （iii）润气过程的性质 将通过针对确定该角色的实验来探索 脉络膜和巩膜补偿近视或远视。