BIOLOGICAL APPLICATIONS OF A NEW MULTILABEL IMAGER

新型多标签成像仪的生物学应用

• 批准号: 3500529
• 负责人: Andrzej K Drukier
• 金额: $ 4.98万
• 依托单位: BIOTRACES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500529
• 关键词: automated data processing; biomedical equipment development; computer human interaction; computer program /software; gamma radiation; image enhancement; nucleic acid sequence; oligonucleotides; personal computers; radiation detector; radiography; radiotracer; rare earth element

BioTraces Inc. (BT Inc.) has developed an ultrasensitive quantitation system which can be used with a large family of isotopes. This system supports label multiplexing; many co-resident isotopes in the family can be quantitated simultaneously, allowing either the pooling of labeled samples or the pooling of distinctly labeled probe reagents for multiple targets. The proposed research will add two dimensional (2D) spatial resolution to the capabilities of the system, in support of nucleic acid quantitation and fractionation, including sequencing. Because of the extensive multiplexing capabilities and superior sensitivity, the proposed technique will greatly increase sample throughput in fractionation/quantitation tasks.

BioTraces公司(BT Inc.)已经开发出了一种超灵敏的定量方法 可以与一大类同位素一起使用的系统。这个系统 支持标签多路复用；家族中许多共存的同位素可以 同时进行定量，允许将标记的 样品或明显标记的探针试剂池用于多个 目标。拟议的研究将增加二维(2D)空间 解决系统支持核酸的能力问题 定量和分馏，包括测序。因为 广泛的多路复用功能和卓越的灵敏度， 建议的技术将极大地提高样品吞吐量 分馏/定量任务。