IMMUNOFLUOROMETRIC ASSAY FOR REPRODUCTIVE HORMONES

描述（改编自申请人摘要）：纳秒 时间分辨荧光检测技术将应用于开发 定量，高灵敏度，免疫荧光测定，同时 在一个样本中测量几种生殖激素。 同相 I这是一个可行性研究，促黄体生成素被用作模型 分析物。 在第二阶段，将研究多种分析物的能力， 临床感兴趣的激素如人绒毛膜促性腺激素（hCG）， 促黄体激素（LH），促卵泡激素（FSH），催乳素， 孕酮雌二醇和睾酮 将开发一个原型，自动随机分析仪和试剂菜单 将扩大到包括上述所有激素。 多个分析物 将通过开发一种原型两种分析物测定法来证明这种能力 用于同时测量LH和FSH。 自动免疫荧光测定系统预期用于 医生的办公室，因此，目标将是设计一个简单的 快速、经济、坚固的系统。

DESCRIPTION (Adapted from the applicant's abstract): A nanosecond time-resolved fluorescence detection technology will be applied to develop quantitative, highly sensitive, immunofluorometric assays for simultaneous measurement of several reproductive hormones in a single sample. In Phase I which was a feasibility study, luteinizing hormone was used as a model analyte. In Phase II multiple analyte capability will be investigated for hormones of clinical interest such as human chorionic gonadotropin (hCG), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, progesterone, estradiol and testosterone. A prototype, automated random analyzer will be developed and reagent menu will be expanded to include all hormones mentioned above. Multiple analyte capability will be demonstrated by developing a prototype two analyte assay for simultaneous measurement of LH and FSH. The automated immunofluorometric assay system is intended for use in physician's office and, therefore, the objective would be to design an easy to use, fast, cost-effective and rugged system.