IMMUNOFLUOROMETRIC ASSAY FOR REPRODUCTIVE HORMONES

创新的纳秒时间分辨荧光检测 技术，具有同时测量多个的潜力 单个样品中的分析物将被应用于开发 新型定量、高灵敏度免疫荧光分析系统 生殖激素。 该项目将需要开发 原型仪器和原型试剂。 在第一阶段黄体化 激素（LH）或人绒毛膜促性腺激素（hCG）将被用作 模型激素以优化灵敏度方面的检测性能， 特异性、简单性、速度和精度；并验证 所提出方法的可行性。 在第二阶段，多种分析物 所提议方法的能力将是生殖激素 临床兴趣。 所提出的免疫测定技术具有 在医生办公室进行非同位素复制的潜力 放射免疫分析的能力，通常只能进行 在医院或大型参考实验室。

An innovative nanosecond time-resolved fluorescence detection technology, with the potential for simultaneously measuring multiple analytes in a single sample, will be applied to the development of a novel quantitative, high-sensitivity immunofluorimetric assay system for reproductive hormones. The project will entail development of both a prototype instrument and prototype reagents. In phase I luteinizing hormone (LH) or human chorionic gonadotropin (hCG) will be employed as a model hormone to optimize assay performance in terms of sensitivity, specificity, simplicity, speed, and precision; and to validate the feasibility of the proposed approach. In phase II, the multiple-analyte capabilities of the proposed approach will be reproductive hormones of clinical interest. The proposed immunoassay technology has the potential to non-isotopically replicate in physician's offices the capabilities of radioimmunoassay, which generally can only be performed in hospitals or large reference laboratories.