IMMUNOFLUOROMETRIC ASSAY FOR REPRODUCTIVE HORMONES

描述(改编自申请人的摘要)：纳秒 时间分辨荧光检测技术将得到应用 定量、高灵敏、免疫荧光同时检测 在单个样本中测量几种生殖激素。同相 一是可行性研究，以促黄体激素为模型 分析物。在第二阶段，将研究多种分析物的能力 临床感兴趣的激素，如人绒毛膜促性腺激素(HCG)， 黄体生成素、卵泡刺激素、催乳素、 黄体酮、雌二醇和睾酮。 将开发一个原型，自动随机分析仪和试剂菜单 将扩大到包括上述所有荷尔蒙。多种分析物 能力将通过开发两种分析物分析的原型来展示 用于同时测定促黄体生成素和卵泡刺激素。 自动免疫荧光分析系统旨在用于 医生的办公室，因此，目标将是设计一种简单的 使用快速、高性价比和坚固耐用的系统。

DESCRIPTION (Adapted from the applicant's abstract): A nanosecond time-resolved fluorescence detection technology will be applied to develop quantitative, highly sensitive, immunofluorometric assays for simultaneous measurement of several reproductive hormones in a single sample. In Phase I which was a feasibility study, luteinizing hormone was used as a model analyte. In Phase II multiple analyte capability will be investigated for hormones of clinical interest such as human chorionic gonadotropin (hCG), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, progesterone, estradiol and testosterone. A prototype, automated random analyzer will be developed and reagent menu will be expanded to include all hormones mentioned above. Multiple analyte capability will be demonstrated by developing a prototype two analyte assay for simultaneous measurement of LH and FSH. The automated immunofluorometric assay system is intended for use in physician's office and, therefore, the objective would be to design an easy to use, fast, cost-effective and rugged system.