GROWTH-ASSOSCIATED PROTEINS

生长相关蛋白

• 批准号: 3504676
• 负责人: LEE D MARGOLIN
• 金额: $ 5万
• 依托单位: CAMBRIDGE NEUROSCIENCE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-20 至 1993-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3504676
• 关键词: Agnatha; biological transport; gel electrophoresis; isoelectric point; molecular weight; nervous system regeneration; neural growth associated protein; protein biosynthesis; radiotracer; spinal cord injury; tissue /cell culture

The objective of this project is to examine the molecular changes that underlie spinal cord regeneration in the lamprey. We will identify and isolate novel growth-associated proteins (GAPS) derived from the central nervous system that are expressed during the course of normal development and subsequent to CNS injury. In higher vertebrates, GAPs are known to be expressed in greater than normal quantities during periods of axonal outgrowth and in response to injury. The search for GAPs derived from the CNS of the sea lamprey represents a potentially valuable avenue of research as lampreys are the only vertebrates to demonstrate functional recovery following complete spinal cord transection. Therefore, the GAPs expressed in this species could provide unique tools for the investigation of the mechanisms of both normal axonal outgrowth and nerve regeneration. During Phase I we will develop a novel in vitro isolated neuraxis preparation that takes advantage of the unique features of lamprey CNS physiology. This preparation will be used in conjunction with two-dimension (2-D) gel electrophoresis of radiolabeled proteins, as it has been employed successfully in the past in the identification and isolation of GAPs found in many other vertebrate species.

这个项目的目的是研究分子变化， 是七鳃鳗脊髓再生的基础 我们将查明和 分离来自中枢神经系统的新的生长相关蛋白（GAPS） 在正常发育过程中表达的神经系统 以及随后的CNS损伤。 在高等脊椎动物中，已知GAP表达于大于 轴突生长期间的正常量和对 损伤 七鳃鳗中枢神经系统GAP的研究 代表了一种潜在的有价值的研究途径，因为七鳃鳗是 只有脊椎动物在完全康复后才能证明功能恢复 脊髓横断 因此，在该物种中表达的GAP 可以提供独特的工具，为调查的机制， 正常轴突生长和神经再生。 在第一阶段，我们将开发一种新的体外分离的轴突， 一种利用七鳃鳗中枢神经系统独特功能的制剂 physiology. 该制剂将与以下药物联合使用： 放射性标记蛋白质的二维（2-D）凝胶电泳， 在过去的鉴定和分离中， 在许多其他脊椎动物物种中发现的缺口。