GROWTH-ASSOSCIATED PROTEINS

这个项目的目标是研究分子变化 七鳃鳗的脊髓再生是其基础。我们将确定和 分离来源于中央的新的生长相关蛋白(GAP) 在正常发育过程中表达的神经系统 以及中枢神经系统损伤后。 在高等脊椎动物中，间隙的表达方式已知大于 轴突生长期间的正常数量和对 受伤。寻找七鳃鳗中枢神经系统发育的缝隙 代表着一种潜在的有价值的研究途径，就像七鳃鳗是 只有脊椎动物在完全恢复后表现出功能恢复 脊髓横断术。因此，在这个物种中表达的差距 可以为研究两者的机制提供独特的工具 正常轴突生长和神经再生。 在第一阶段，我们将开发一种新的体外分离的神经轴 利用七鳃鳗中枢神经系统的独特功能进行准备 生理学。该制剂将与以下药物联合使用 放射性标记蛋白质的二维(2-D)凝胶电泳 过去曾成功地应用于鉴定和隔离 在许多其他脊椎动物物种中发现的缺口。

The objective of this project is to examine the molecular changes that underlie spinal cord regeneration in the lamprey. We will identify and isolate novel growth-associated proteins (GAPS) derived from the central nervous system that are expressed during the course of normal development and subsequent to CNS injury. In higher vertebrates, GAPs are known to be expressed in greater than normal quantities during periods of axonal outgrowth and in response to injury. The search for GAPs derived from the CNS of the sea lamprey represents a potentially valuable avenue of research as lampreys are the only vertebrates to demonstrate functional recovery following complete spinal cord transection. Therefore, the GAPs expressed in this species could provide unique tools for the investigation of the mechanisms of both normal axonal outgrowth and nerve regeneration. During Phase I we will develop a novel in vitro isolated neuraxis preparation that takes advantage of the unique features of lamprey CNS physiology. This preparation will be used in conjunction with two-dimension (2-D) gel electrophoresis of radiolabeled proteins, as it has been employed successfully in the past in the identification and isolation of GAPs found in many other vertebrate species.