HEAVY CLUSTER IMPACT DESORPTION OF LARGE MOLECULES

大分子的重簇冲击解吸

• 批准号: 3508031
• 负责人: JOHN F MAHONEY
• 金额: $ 25.62万
• 依托单位: PHRASOR SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508031
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; chemical models; high energy particle; ionization; macromolecule; mass spectrometry; particle beam; solvents

The long term objectives of the proposed research are to develop, manufacture and market new instrumentation for the sputtering of large, fragile, molecules. This new technology, called massive cluster impact mass spectrometry(MCIMS), is based on using massive, multiply charged, organic clusters as the primary beam for desorbing sample ions entrained in liquid matrices. We propose to meet these objectives by developing a bipolar (positive or negative) massive cluster ion beam source capable of impacting glycerol matrices with energies/nucleon in the range 0.01 eV/amu less than E/N less than 1.0 eV/amu providing an extremely soft method for sputtering intact molecules. The sputtering process, governed by shock wave phenomenon, is expected to provide enhanced secondary ion yields with significantly reduced ionization and radiation damage of matrix material. With these benefits, massive cluster impact spectra show lower chemical noise background and improved signal-to- noise ratios. In addition to the desorption of large biomolecules, this new "soft" ionization method should prove advantageous for the mass spectral analyses of mixtures, the study of solution phase chemistry and the investigation of fundamental aspects of desorption processes.

拟议研究的长期目标是开发， 制造和销售用于溅射大型， 脆弱的分子 这项新技术，叫做大规模集群撞击 质谱法（MCIMS）是基于使用大量的，多电荷的， 以有机团簇作为主束， 在液体基质中。 我们建议通过发展 一种双极（正或负）大质量团簇离子束源， 以0.01的能量/核子撞击甘油基质 eV/amu小于E/N小于1.0eV/amu， 溅射完整分子的方法。 溅射过程， 受冲击波现象支配，预计将提供增强的 二次离子产率，电离和辐射显著降低 基体材料的损坏。 有了这些好处， 光谱显示较低化学噪声背景和改善的信号- 噪声比 除了大生物分子的解吸附外，这 新的“软”电离方法应该证明有利于质量 混合物的光谱分析，溶液相化学的研究， 对解吸过程的基本方面的研究。