RAPID,SENSITIVE SEQUENCING OF 2-D GEL SEPARATED PROTEINS

该提案描述了一项新技术的开发，以获得 通过 2D 凝胶电泳分离的蛋白质的序列信息。这个 仪器将能够同时对多个样本进行测序， 比现有技术获得的灵敏度更高。这些 功能将大大提高许多测序的效率 2D 凝胶电泳后可能存在的蛋白质 复杂蛋白质混合物的分离，例如用于分析 细胞裂解物中存在的蛋白质。区分的关键组件 商业模式中拟议的工具是：利用 用于精确输送小体积的压电液滴发生器 试剂；利用二次飞行质谱仪 测序产物的检测；并且，测序能力 同时进行多个样品。

This proposal describes the development of a new technology to obtain sequence information on proteins separated by 2D-gel electrophoresis. This instrument will be capable of sequencing multiple samples simultaneously, with greater sensitivity than that obtained with current technology. These features will greatly increase the efficiency of sequencing the many proteins that may be present following the 2D-gel electrophoretic separation of complex protein mixtures, for example for analysis of the proteins present in a cellular lysate. The key components that distinguish the proposed instrument from commercial models are: utilization of piezoelectric droplet generators for the precise delivery of small volumes of reagents; utilization of a two-of-flight mass spectrometer for detection of the sequencing products; and, the capacity for sequencing multiple samples simultaneously.