RAPID, SENSITIVE SEQUENCING OF 2-D GEL SEPARATED PROTEIN

该提案描述了一种新技术的开发， 通过2D凝胶电泳分离的蛋白质的序列信息。 该仪器将能够对多个样本进行测序 同时，具有比电流更高的灵敏度 技术. 这些特性将大大提高 对2D凝胶后可能存在的许多蛋白质进行测序 复杂蛋白质混合物的电泳分离，例如 细胞裂解物的分析。 这种定序器设计在以下方面是独特的： 溶剂和试剂输送阀系统的概念， 样品室和转化瓶位于单独的旋转盘上， 从一个输送阀旋转到下一个输送阀， 多个样品同时进行，并利用飞行时间 用于检测测序产物的质谱仪。 拟议的商业应用：成功完成拟议的 研究将最终在商业上可用，自动化，多- 样品，2D-Gel蛋白质测序仪单元用于生物化学工业。

This proposal describes the development of a new technology to obtain sequence information on proteins separated by 2D-gel electrophoresis. This instrument will be capable of sequencing multiple samples simultaneously, with greater sensitivity than that obtained with current technology. These features will greatly increase the efficiency of sequencing the many proteins that may be present following the 2D-gel electrophoretic separation of complex protein mixtures, for example the analysis of cellular lysate. This sequencer design is unique in terms of the solvent and reagent delivery valve system, the concept of having the samples chambers and conversion flasks on separate, rotating discs that rotate from one delivery valve to the next, the ability to sequence multiple samples simultaneously, and the utilization of a time-of-flight mass spectrometer for detection of the sequencing products. PROPOSED COMMERCIAL APPLICATION: Successful completion of the proposed research will culminate in commercially available, automated, multi- sample, 2D-Gel protein sequencer unit for the biochemical industry.