RAPID, SENSITIVE SEQUENCING OF 2-D GEL SEPARATED PROTEIN

这份提案描述了一种新技术的发展，以获得 双向凝胶电泳法分离蛋白质的序列信息。 这台仪器将能够对多个样本进行测序 同时，比用电流获得的灵敏度更高 技术这些功能将极大地提高 对2D-Gel后可能存在的许多蛋白质进行测序 复杂蛋白质混合物的电泳法分离，例如 细胞裂解物的分析。这种定序器的设计在以下方面是独一无二的 溶剂和试剂输送阀门系统，具有 在分开的旋转圆盘上的样品室和转化瓶 从一个传送阀到另一个传送阀旋转，能够排序 同时多个样本，以及飞行时间的利用 用于检测测序产物的质谱仪。 拟议的商业应用：成功完成拟议的 研究将最终实现商业化、自动化、多功能化 样品，2D-Gel蛋白质测序仪，用于生化工业。

This proposal describes the development of a new technology to obtain sequence information on proteins separated by 2D-gel electrophoresis. This instrument will be capable of sequencing multiple samples simultaneously, with greater sensitivity than that obtained with current technology. These features will greatly increase the efficiency of sequencing the many proteins that may be present following the 2D-gel electrophoretic separation of complex protein mixtures, for example the analysis of cellular lysate. This sequencer design is unique in terms of the solvent and reagent delivery valve system, the concept of having the samples chambers and conversion flasks on separate, rotating discs that rotate from one delivery valve to the next, the ability to sequence multiple samples simultaneously, and the utilization of a time-of-flight mass spectrometer for detection of the sequencing products. PROPOSED COMMERCIAL APPLICATION: Successful completion of the proposed research will culminate in commercially available, automated, multi- sample, 2D-Gel protein sequencer unit for the biochemical industry.