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HEAVY CLUSTER IMPACT DESORPTION OF LARGE MOLECULES

大分子的重簇冲击解吸

基本信息

批准号：
2183304
负责人：
JOHN F MAHONEY
金额：
$ 24.38万
依托单位：
PHRASOR SCIENTIFIC, INC.
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-01-01 至 1995-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2183304
关键词：
bioengineering /biomedical engineering biomedical equipment development chemical models high energy particle ionization macromolecule mass spectrometry particle beam solvents

项目摘要

The long term objectives of the proposed research are to develop, manufacture and market new instrumentation for the sputtering of large, fragile, molecules. This new technology, called massive cluster impact mass spectrometry(MCIMS), is based on using massive, multiply charged, organic clusters as the primary beam for desorbing sample ions entrained in liquid matrices. We propose to meet these objectives by developing a bipolar (positive or negative) massive cluster ion beam source capable of impacting glycerol matrices with energies/nucleon in the range 0.01 eV/amu less than E/N less than 1.0 eV/amu providing an extremely soft method for sputtering intact molecules. The sputtering process, governed by shock wave phenomenon, is expected to provide enhanced secondary ion yields with significantly reduced ionization and radiation damage of matrix material. With these benefits, massive cluster impact spectra show lower chemical noise background and improved signal-to- noise ratios. In addition to the desorption of large biomolecules, this new "soft" ionization method should prove advantageous for the mass spectral analyses of mixtures, the study of solution phase chemistry and the investigation of fundamental aspects of desorption processes.

拟议研究的长期目标是开发，制造和销售用于大型、脆弱, 分子. 这种新技术，叫做海量集群冲击质谱法 (MCIMS)，基于使用大量、多电荷、有机团簇作为解吸夹带样品离子的主光束在液体基质中。我们建议通过开发来实现这些目标双极（正或负）大量簇离子束源能量/核子在 0.01 范围内对甘油基质的影响 eV/amu 小于 E/N 小于 1.0 eV/amu，提供极其柔软的溅射完整分子的方法。溅射工艺，受冲击波现象控制，预计将提供增强二次离子产量显着降低电离和辐射基体材料的损坏。凭借这些优势，巨大的集群影响光谱显示较低的化学噪声背景和改进的信号比噪声比。除了大生物分子的解吸之外，这新的“软”电离方法应该被证明对质量有利混合物的光谱分析、溶液相化学的研究和解吸过程基本方面的研究。