HIGH-MASS MOLECULAR ION ELECTROHYDRODYNAMIC EMISSION

高质量分子离子电动发射

• 批准号: 3509385
• 负责人: JOHN F MAHONEY
• 金额: $ 25.12万
• 依托单位: PHRASOR SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3509385
• 关键词: chemical models; cold temperature; electric field; electronic spectra; evaporation; head movements; ionization; ions; macromolecule; mass spectrometry; model design /development; molecular weight; nontherapeutic iontophoresis; proteins; solvents

The long term objective of this application is to develop instrumentation for the mass analysis of biological macromolecules in the mass range 10,000 to 100,000 amu. The specific aim of Phase II is to demonstrate the emission and detection of biological sample ions to MW 11,500 using an electrohydrodynamic (EHD) ionization source mounted on a JEOL double focusing mass spectrometer. The methodology for meeting this goal includes a continuation of Phase I studies which examined sample ion desolvation, solvent chemistry and methods for handling small and valuable samples. Enhancement of the intensity of molecular ion emission by cooling the EHD ion source and improved ion transmission by adding beam optics will be pursued. Particular attention will be focused on the demonstrated capability of the EHD ionization process to produce doubly and triply charged ions for extending the detectable mass range of sample ions. Aqueous or high volatility solvents will be explored and characterized for their potential of achieving complete sample desolvation and reducing the complexities in high mass ion spectra observed with glycerol. The commercial application of EHD ion source development, coupled to a matching mass analyzer, will impact the biotechnology field by introducing a new, more accurate method of high mass molecular weight determinations for proteins and other macromolecular biological compounds.

该应用程序的长期目标是开发 生物大分子质量分析仪器 质量范围为 10,000 至 100,000 amu。 阶段的具体目标 二是演示生物的发射和检测 使用电流体动力学 (EHD) 将样品离子测量到 MW 11,500 安装在 JEOL 双聚焦质量上的电离源 光谱仪。 实现这一目标的方法包括 继续第一阶段研究，检查样品离子 去溶剂化、溶剂化学以及处理小分子和小分子的方法 有价值的样品。 分子离子强度增强 通过冷却 EHD 离子源和改进的离子进行发射 将寻求通过添加光束光学器件进行传输。 特别的 注意力将集中在 EHD 所展示的能力上 电离过程产生双电荷和三电荷离子 扩展样品离子的可检测质量范围。 水性或 将探索和表征高挥发性溶剂 它们实现样品完全去溶剂化的潜力以及 降低观察到的高质量离子光谱的复杂性 甘油。 EHD离子源的商业化应用 开发，加上匹配的质量分析仪，将影响 生物技术领域通过引入一种新的、更准确的方法 蛋白质和其他物质的高质量分子量测定 高分子生物化合物。