CONFOCAL LASER SCANNING MICROSCOPE FACILITY

共焦激光扫描显微镜设备

基本信息

批准号：
3522015
负责人：
ANDREA J ELBERGER
金额：
$ 15.6万
依托单位：
UNIVERSITY OF TENNESSEE HEALTH SCI CTR
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 1994-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3522015
关键词：
DVD /CD ROM biomedical equipment purchase biomedical equipment resource central nervous system computer data analysis computer network computer program /software computer system hardware confocal scanning microscopy fluorescence microscopy image processing lasers neuroanatomy

项目摘要

Purchase of a Confocal Laser Scanning Microscope (CLSM) system, to be installed in the Department of Anatomy and Neurobiology at the University of Tennessee, Memphis is proposed. The CLSM will facilitate morphological investigation of central nervous system (CNS) function and structure by providing types of analyses that cannot be done with currently available light or electron microscopic techniques. Ongoing NIH-funded studies in the Department utilize fluorescent and non- fluorescent pathway tracers, cell markers, and histochemical labels throughout the CNS. Analyses include identification, localization, characterization and measurement of related CNS structures. The Bio-Rad MRC-600 CLSM system features an Argon/Krypton laser with wavelengths of 488, 568 and 647 nm permitting single-triple labeling, and 2 detection channels for simultaneous double label imaging; each channel features a selectable aperture and analog adjustment of signal dynamic range. The system will be operated by a Gateway PC/AT 486 compatible computer; an external WORM drive with separate cartridges for each User will provide long-term storage. The MS-DOS system will operate Bio-Rad's user-friendly windows-based software for acquisition, processing, and quantification of images in 2 and 3 dimensions. Additional software is available for 3-dimensional rotation and quantification. A epifluorescence microscope with several filter sets and a range of high numerical aperture objectives will be purchased. An identical second Bio-Rad computer workstation with a WORM drive will serve the 4 following functions: providing a training facility, transmitting files via Ethemet, permitting off-line data analysis for non-networked Users, and permitting off-line data analysis for Users without PC/AT computers. This will permit the operating computer with confocal image capture system to remain available for the primary purpose of image generation. The system package includes installation, training, software updates and a 1 year warranty. The second Bio-Rad computer workstation will be networked to a subnet of the Ethernet backbone at The University of Tennessee, Memphis that already transmits video images from an electron microscope, a Macintosh IIci image analysis workstation using programs such as the NIH IMAGE software, and a PC/AT compatible workstation using the Eutectics Neuron Tracing System; networking will allow Users to interface confocal images with a variety of software packages for image analysis as well as presentation.

购买共聚焦激光扫描显微镜（CLSM）系统，在大学的解剖学和神经生物学系安装提出了孟菲斯的田纳西州。 CLSM将有助于中枢神经系统（CNS）功能和通过提供无法完成的分析的类型来结构当前可用的光或电子显微镜技术。正在进行 NIH资助的部门利用荧光和非 - 荧光途径示踪剂，细胞标记和组织化学标记在整个中枢神经系统中。分析包括识别，本地化，相关CNS结构的表征和测量。 Bio-Rad MRC-600 CLSM系统具有氩/krypton激光器波长为488、568和647 nm，允许单三个标记，并且 2个同时双重标签成像的检测通道；每个通道具有可选的光圈和信号动态信号的模拟调整范围。该系统将由网关PC/AT 486兼容操作电脑;每个用户的外部蠕虫驱动器带有单独的墨盒将提供长期存储。 MS-DOS系统将运行Bio-Rad的用户友好的基于Windows的软件可用于获取，处理和在2和3维图中的图像进行量化。其他软件是可用于三维旋转和定量。一个带有几个滤镜和一定范围的高荧光显微镜将购买数值孔径目标。带有蠕虫驱动器的第二个与蠕虫驱动器相同的第二次生物拉德计算机工作站服务以下4个功能：提供培训设施，通过Ethemet传输文件，允许离线数据分析非网络用户，并允许用户离线数据分析没有PC/在计算机上。这将允许使用共聚焦图像捕获系统将用于主要目的图像生成。系统软件包包括安装，培训，软件更新和1年保修。第二个Bio-Rad计算机工作站将与田纳西大学孟菲斯大学的以太网骨干已经从电子显微镜（Macintosh）传输视频图像 IICI图像分析工作站使用诸如NIH图像之类的程序软件和PC/在兼容工作站使用Eutectics神经元跟踪系统；网络将允许用户接口共焦图像带有各种软件包，用于图像分析以及推介会。