CONFOCAL MICROSCOPE SYSTEM

共焦显微镜系统

• 批准号: 3521735
• 负责人: SANSAR C SHARMA
• 金额: $ 17.3万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-26 至 1994-05-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521735
• 关键词: DVD /CD ROM; biomedical equipment purchase; biomedical equipment resource; computer data analysis; computer program /software; confocal scanning microscopy; image processing; lasers; personal computers

This proposal requests funds to purchase a Bio-Rad confocal system which will attach to a Nikon Optiphot microscope. The standard configuration of this instrument will be purchased with the addition of a second laser and a software analysis package which are justified. Administrative mechanisms are described. Eight separate laboratories have proposed experiments using this instrument. These projects include: a) localization of labelled intracellular molecules, including double labelling experiments which will distinguish the locations of pair of molecules; b) three dimensional reconstruction of neurons and tissue interfaces; c) nerve tracing during development; and d) imaging of spatially and temporally resolved ion transients using fluorescent indicators. The microscope will become one of the core facilities at New York Medical College, serving the entire basic medical science community.

该提案要求资金购买一个生物-RAD共聚焦系统 将连接到尼康的选择显微镜上。 标准配置 该乐器将在添加第二激光的情况下购买 以及合理的软件分析软件包。 行政 描述了机制。 已经提出了八个独立的实验室 实验使用此仪器。 这些项目包括：a） 标记的细胞内分子的定位，包括双 标记实验将区分一对的位置 分子； b）神经元和组织的三维重建 接口； c）发育过程中的神经追踪； d）成像 使用荧光在空间和时间上分辨的离子瞬变 指标。 显微镜将成为新的核心设施之一 约克医学院，为整个基础医学社区服务。