ELECTROSPRAY IONIZATION MASS SPECTROMETRY SYSTEM

电喷雾电离质谱系统

• 批准号: 3521352
• 负责人: JIRI JONAS
• 金额: $ 40万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-19 至 1993-05-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521352
• 关键词: biomedical equipment purchase; mass spectrometry

A state-of-the-art triple quadrupole mass spectrometer with an electrospray ionization source will be employed for use in the School of Chemical Sciences Mass Spectrometry Laboratory to support the research of the faculty, postdoctorates, and graduate students in Chemistry, Biochemistry, and the School of Life Sciences. The system will be employed for the study of trace amounts of biological macromolecules of high molecular weight. Major users of the electrospray ionization system will be J. E. Cronan, Jr., R. B. Gennis, R. I. Gumport, A. Jonas, K.L. Rinehart, S. G. Sligar, and K. S. Suslick in their studies of post-translational modifications of ACP from E. coli; membrane enzymes in respiratory electron transport reactions; recognition of sequence-specific protein-DNA interactions; structure and function of plasma high density lipoproteins; structure and chemistry of antibiotics, peptides, and related compounds; chemical mechanism of cytochrome P-450 catalysis; synthetic analogs of heme proteins; Additional users will be A. R. Crofts, A. L. DeVries, S. Kyin, and G. W. Ordal in studies of structure/function relationships in photosynthetic enzyme conversions; the role of antifreeze proteins in polar fishes; the determination of molecular weights and sequences of genetically engineered proteins and DNA molecules; the study of sensory transduction by bacterial chemotaxis.

配备电喷雾的最先进的三重四极杆质谱仪 电离源将用于化学学院 科学质谱实验室支持的研究 化学、生物化学、 和生命科学学院。 该系统将用于研究 微量的高分子量生物大分子。 电喷雾电离系统的主要用户是 J. E. Cronan， Jr.、R. B. Gennis、R. I. Gumport、A. Jonas、K.L.莱因哈特，S.G.斯利加尔， 和 K. S. Suslick 在他们的翻译后修饰研究中 来自大肠杆菌的 ACP；呼吸电子传递中的膜酶 反应；识别序列特异性蛋白质-DNA 相互作用； 血浆高密度脂蛋白的结构和功能；结构和 抗生素、肽和相关化合物的化学；化学 细胞色素P-450催化机制；血红素的合成类似物 蛋白质；其他用户包括 A. R. Crofts、A. L. DeVries、S. Kyin、 和 G. W. Ordal 的结构/功能关系研究 光合酶的转化；抗冻蛋白在极性中的作用 鱼；分子量和遗传序列的测定 工程蛋白质和 DNA 分子；感觉传导的研究 细菌趋化性。