HIGH PRESSURE NMR STUDIES OF PROTEINS
蛋白质的高压核磁共振研究
基本信息
- 批准号:2181382
- 负责人:
- 金额:$ 20.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-07-01 至 1998-07-31
- 项目状态:已结题
- 来源:
- 关键词:biomedical equipment development biophysics chemical kinetics chemical stability circular dichroism conformation fatty acid binding protein hydropathy lasers lysozyme mechanical pressure molecular dynamics mutant myoglobin nuclear magnetic resonance spectroscopy nuclease pancreatic ribonuclease photochemistry protein denaturation protein folding protein structure temperature thermodynamics
项目摘要
The long-term objective of the proposed research is to contribute to the
understanding of the stability, folding intermediates, and dynamics of
proteins by using pressure as the fundamental thermodynamic variable.
Unique high pressure equipment, suitable for measurements from 0.1 MPa (l
bar) to l GPa (10 kbar) and from -25 degrees C to lOO degrees C, will be
used in conjunction with a variety of l-D and 2-D high resolution NMR
techniques to study the effects of pressure on the unfolding/folding
equilibria, conformational states of native and compact denatured states,
and the dynamic properties of several model proteins. In addition, the
cold-denaturation behavior of these proteins will be studied by taking
advantage of the depression of the freezing point of water by pressure.
The pressure, cold, heat, and chemically denatured states of the model
proteins will be examined and compared, with special emphasis on the
compact denatured states observed by cold and pressure denaturation. The
model proteins selected for these studies include hen egg-white lysozyme,
bacteriophage T4 lysozyme, horse myoglobin, sperm-whale myoglobin,
staphylococcal nuclease, and bovine pancreatic ribonuclease A, as well as
selected mutants of T4 lysozyme, sperm-whale myoglobin, staphylococcal
nuclease, and bovine pancreatic ribonuclease A.
To complement the NMR measurements at high pressure, especially on the
compact denatured states of the model proteins and their mutants, circular
dichroism (CD) and laser-photo chemically induced dynamic nuclear
polarization (CIDNP) experiments will be performed. High-pressure CD
cells and CIDNP probes will be designed and constructed.
拟议研究的长期目标是促进
了解的稳定性,折叠中间体,和动力学的
用压力作为基本热力学变量来计算蛋白质。
独特的高压设备,适用于从0.1 MPa(l)
巴)至lGPa(10千巴)以及从-25摄氏度至100摄氏度,将是
与各种1-D和2-D高分辨率NMR结合使用
研究压力对展开/折叠影响的技术
平衡,天然和致密变性状态的构象状态,
以及几种模型蛋白质的动力学性质。 此外该
这些蛋白质的冷变性行为将被研究,
水的凝固点通过压力降低的优点。
模型的压力、冷、热和化学变性状态
蛋白质将被检查和比较,特别强调
通过冷变性和压力变性观察到的致密变性状态。的
选择用于这些研究的模型蛋白包括鸡蛋白溶菌酶,
噬菌体T4溶菌酶,马肌红蛋白,抹香鲸肌红蛋白,
葡萄球菌核酸酶和牛胰腺核糖核酸酶A,以及
T4溶菌酶、抹香鲸肌红蛋白、葡萄球菌
核酸酶和牛胰腺核糖核酸酶A。
为了补充高压下的NMR测量,特别是在
模型蛋白及其突变体的紧凑变性状态,环状
二向色性(CD)和激光光化学诱导动态核
极化(CIDNP)实验。 高压CD
细胞和CIDNP探针将被设计和构建。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('JIRI JONAS', 18)}}的其他基金
HYDROGEN EXCHANGE BEHAVIOR OF PRESSURE DENATURED RIBONUCLEASE BY 2D COSY NMR
2D Cosy NMR 测定压力变性核糖核酸酶的氢交换行为
- 批准号:
6253376 - 财政年份:1997
- 资助金额:
$ 20.86万 - 项目类别:
HIGH PRESSURE NMR STUDIES OF PROTEINS AND MEMBRANES
蛋白质和膜的高压核磁共振研究
- 批准号:
3301009 - 财政年份:1989
- 资助金额:
$ 20.86万 - 项目类别:
HIGH PRESSURE NMR STUDIES OF PROTEINS AND MEMBRANES
蛋白质和膜的高压核磁共振研究
- 批准号:
3301008 - 财政年份:1989
- 资助金额:
$ 20.86万 - 项目类别:
HIGH PRESSURE NMR STUDIES OF PROTEINS AND MEMBRANES
蛋白质和膜的高压核磁共振研究
- 批准号:
3301010 - 财政年份:1989
- 资助金额:
$ 20.86万 - 项目类别:
HIGH PRESSURE NMR STUDIES OF PROTEINS AND MEMBRANES
蛋白质和膜的高压核磁共振研究
- 批准号:
3301007 - 财政年份:1989
- 资助金额:
$ 20.86万 - 项目类别:
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