DETECTION OF HEPATITIS B VIRUS DNA USING THE POLYMERASE CHAIN REACTION

使用聚合酶链反应检测乙型肝炎病毒 DNA

• 批准号: 3809722
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809722
• 关键词: antiviral antibody; chronic disease /disorder; communicable disease diagnosis; diagnosis design /evaluation; hepatitis B; hepatitis B antigens; hepatitis B virus group; human tissue; immunologic assay /test; liver disorder diagnosis; molecular pathology; natural gene amplification; nucleic acid hybridization; nucleic acid probes; polymerase chain reaction; serum; virus DNA; virus classification

Standard methods of virus DNA detection using the polymerase chain reaction (PCR) can be time consuming and involve multiple steps during which contamination with exogenous DNA often occurs. Therefore, we developed a simplified method for detecting hepatitis B virus (HBV) DNA in serum. The main advantages of this method are that it can be performed rapidly, it consists of only a few steps, and has a false positive rate of <0.1% in our laboratory. In testing serum from 84 human patients, we found HBV DNA in all patients who had HBsAg and hepatitis B e antigen (HBeAg) in serum and in 64% of the patients who had HBsAg, but not HBeAg, in serum. Also, 3 of 11 patients who were chronic HBV carriers and who subsequently lost HBsAg were found to have HBV DNA in serum. In contrast, all patients who lost HBsAg after acute HBV infection or those classified as having non-A, non-B hepatitis were negative for HBV DNA. Thus, the modified PCR technique is a sensitive and rapid method for detecting HBV DNAcontaining virions in serum.

用聚合酶链反应检测病毒DNA的标准方法