WOODCHUCK VIRUS--MOLECULAR BIOLOGICAL STUDIES

土拨鼠病毒——分子生物学研究

• 批准号: 3790721
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790721
• 关键词: animal viral hepatitis; chronic disease /disorder; duck hepatitis B virus; gene rearrangement; genetic promoter element; genetic strain; genome; liver neoplasms; marmots; mutant; open reading frames; virus assembly; virus genetics; virus replication

The woodchuck hepatitis virus (WHV) is taxonomically and serologically related to the hepatitis B virus (HBV). Infection with each of these viruses is associated with acute and chronic hepatitis and hepatic cell carcinoma in their respective hosts and these associations appear to be etiological in nature. Thus, WHV infection of woodchucks provides a relevant and convenient model for understanding HBV infections of humans. The following experiments were performed: (1) detection of WHV mutants in chronically infected woodchucks, (2) mutant WHV genomes in serum were found to resemble rearranged viral integrants in hepatocellular carcinoma, (3) analysis of the X gene promoter, (4) newly identified open reading frames of WHV are not essential for virus replication, (5) mutational analysis of the WHV X gene, and (6) analysis of a WHV mutant with a defective precore gene.

土拨鼠肝炎病毒（WHV）在分类学和血清学上是 与B型肝炎病毒（HBV）有关。 感染这些病毒 病毒与急性和慢性肝炎及肝细胞 癌在各自的宿主，这些协会似乎是 本质上是病因学的。 因此，土拨鼠的WHV感染提供了一种 相关和方便的模型，了解人类HBV感染。 进行了以下实验：（1）检测WHV突变体， 慢性感染的土拨鼠，（2）在血清中发现突变的WHV基因组 类似于肝细胞癌中重排的病毒整合体，（3） X基因启动子的分析，（4）新鉴定的开放阅读框 的突变分析， WHV X基因，和（6）具有缺陷前C的WHV突变体的分析 基因