MOLECULAR AND COMPUTER ANALYSIS OF THE HEPATITIS B VIRUS GENOME

乙型肝炎病毒基因组的分子和计算机分析

• 批准号: 3803224
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3803224
• 关键词: animal tissue; antiviral antibody; duck hepatitis B virus; hepatitis B; hepatitis B virus group; human tissue; nucleic acid sequence; polymerase chain reaction; serology /serodiagnosis; serum; virus DNA

The technique of amplifying DNA using the polymerase chain reaction (PCR) assay is a powerful method for detecting low levels of DNA. We developed a simple and rapid PCR method for the detection of hepatitis B virus (HBV) DNA in serum. In testing serial samples from five chimpanzees experimentally infected with HBV, we found that HBV DNA was detected 2-3 weeks before the appearance of hepatitis B surface antigen (HBsAg), and continued to be detectable 1-3 weeks after the production of antibody to HBsAg. Sera from 98 patients at various stages of chronic HBV infection were also studied. Patients were divided into three groups according to their HBsAg and hepatitis Be antigen (HBeAg) status. Group I patients were positive for both HBsAg and HBeAg in the serum. PCR analysis demonstrated that all 31 (100%) of these patients possessed serum HBV DNA. Group II patients were positive for HBsAg and negative for HBeAg. Analysis showed that 36 of 46 (78%) of these patients possessed serum HBV DNA. Group III patients were former chronic HBV carriers but subsequently lost HBsAg during follow up. In this group 5 of 21 (24%) were found to harbor HBV DNA sequences in the serum. Detection of HBV DNA by PCR analysis is a valuable method for detecting the presence of DNA-containing virions in serum.

使用聚合酶链式反应扩增 DNA 的技术 (PCR) 检测是检测低水平 DNA 的有效方法。 我们 开发了一种简单快速的PCR检测乙型肝炎的方法 血清中的病毒（HBV）DNA。 在测试五个系列样品时 我们在黑猩猩实验中感染了乙肝病毒，发现乙肝病毒DNA 乙型肝炎表面抗原出现前2-3周检测 (HBsAg)，并在生产后1-3周继续可检测到 HBsAg 抗体。 98名不同阶段患者的血清 还对慢性乙型肝炎病毒感染进行了研究。 患者被分为 根据 HBsAg 和 Be 型肝炎抗原 (HBeAg) 分为三组 地位。 第一组患者的 HBsAg 和 HBeAg 均呈阳性 血清。 PCR 分析表明，所有 31 名患者 (100%) 拥有血清 HBV DNA。 第 II 组患者 HBsAg 呈阳性且 HBeAg 阴性。分析显示，其中 46 个中的 36 个（78%） 患者血清中含有 HBV DNA。 第三组患者是前 慢性 HBV 携带者，但随后在随访期间失去 HBsAg。 在 这组 21 人中有 5 人 (24%) 被发现在 血清。 PCR 分析检测 HBV DNA 是一种有价值的方法。 检测血清中是否存在含有 DNA 的病毒粒子。