MOLECULAR AND COMPUTER ANALYSIS OF THE HEPATITIS B VIRUS GENOME

乙型肝炎病毒基因组的分子和计算机分析

• 批准号: 3818297
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818297
• 关键词: DNA directed RNA polymerase; RNA directed DNA polymerase; Retroviridae disease; computer data analysis; duck hepatitis B virus; genome; nucleic acid hybridization; nucleic acid sequence; virus DNA; virus envelope; virus genetics; virus replication

The purpose of this project is to study the HBV genome by computer and molecular hybridization analysis. Our data suggest that HBV and retroviruses evolved from a common ancestor. This is a significant finding because it demonstrates the relatedness of two diverse virus families. We find that: (1) both HBV and retroviruses utilize a unique mechanism of genome replication by reverse transcription of RNA; (2) highly conserved regions of HBV proteins share considerable homology with retrovirus nucleocapsid, protease, and polymerase proteins; (3) the HBV polymerase shares organizational similarities with the polymerase gene sequence of retroviruses and retrovirus-like genetic elements; (4) genetic analysis and low stringency blot hybridization indicates that HBV DNA sequences are most closely related to MLV DNA sequences; (5) HBV possesses two previously undiscovered open reading frames which may represent genuine gene sequences. Interestingly, one of the new open reading frames is located on the DNA plus strand of the double-stranded virus genome. This also appears to be a unique feature of retroviruses that encode proteins involved in transcription activation because we find a plus strand DNA ORF in the human immunodeficiency virus genome, and in the genomes of related retroviruses of humans and animals. Overall, HBV and retorviruses share a common mechanism of genome replication, possess homologous nucleotide and amino acid, and have genomes that share a similar organization of genes. Therefore, it is probable that these virus families evolved from a common ancestral virus.

该项目的目的是通过计算机研究乙型肝炎病毒基因组 和分子杂交分析。 我们的数据表明，乙型肝炎 逆转录病毒是从共同的祖先进化而来的。 这是一个 重大发现，因为它证明了两者的相关性 不同的病毒家族。 我们发现： (1) HBV 和 逆转录病毒利用独特的基因组复制机制 RNA逆转录； (2) HBV高度保守区域 蛋白质与逆转录病毒核衣壳具有相当大的同源性， 蛋白酶和聚合酶蛋白； (3) HBV聚合酶股 与聚合酶基因序列的组织相似性 逆转录病毒和逆转录病毒样遗传元件； (4)遗传 分析和低严格印迹杂交表明 HBV DNA序列与MLV DNA序列关系最为密切； (5) HBV拥有两个以前未被发现的开放阅读框 可能代表真实的基因序列。 有趣的是，其中之一 新的开放阅读框位于DNA正链上 双链病毒基因组。 这似乎也是一个独特的 编码参与的蛋白质的逆转录病毒的特征 转录激活是因为我们发现了一个正链 DNA ORF 人类免疫缺陷病毒基因组，以及 人类和动物的相关逆转录病毒。 总体而言，乙肝病毒和 逆转录病毒具有共同的基因组复制机制， 具有同源的核苷酸和氨基酸，并具有以下基因组： 具有相似的基因组织。 因此，很可能 这些病毒家族是从共同祖先病毒进化而来的。