MOLECULAR AND COMPUTER ANALYSIS OF THE HEPATITIS B VIRUS GENOME

乙型肝炎病毒基因组的分子和计算机分析

• 批准号: 3746568
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3746568
• 关键词: computer assisted sequence analysis; gene mutation; genetic promoter element; genome; hepatitis B virus group; microorganism culture; mutant; nucleic acid sequence; virus genetics

Hepatitis B virus (HBV) is the prototype of the hepadnavirus family, which includes hepatotropic viruses that infect at least six separate species. Infection with HBV causes polymorphic liver diseases including acute or fulminant hepatitis, chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Over 300 million people throughout the world are chronically infected with HBV and are at high risk of developing hepatocellular carcinoma, one of the world's most prevalent forms of cancer. Within the last year we pursued two avenues of investigation. First, we continued our characterization of a putative neutralization escape mutant of HBV that emerged in individuals who were vaccinated against hepatitis B. Two licensed vaccines were investigated for their ability to protect chimpanzees against challenge with the S gene mutant. Both recombinant vaccines provided protection against challenge with the mutant suggesting that properly vaccinated individuals are not at risk of infection by the S gene mutant virus. Second, a bi-directional promoter complex was iden- tified within the X gene of HBV further emphasizing the complex genetic organization of this virus.

乙肝病毒(HBV)是肝炎病毒家族的原型， 包括至少感染六种不同病毒的嗜肝病毒 物种。感染乙肝病毒会导致多种肝病，包括 急性或暴发性肝炎、慢性肝炎、肝硬变和 肝细胞癌。全世界有超过3亿人 长期感染乙肝病毒，并处于发展的高危状态 肝细胞癌是世界上最常见的癌症之一 癌症。 在过去的一年里，我们寻求了两种调查途径。首先，我们 继续我们对推测的中和逃逸突变体的描述 在接种了肝炎疫苗的个人中出现的乙肝病毒 B.对两种获得许可的疫苗进行了保护能力的调查 用S基因突变体对抗黑猩猩的挑战。都是重组的 疫苗提供了对挑战的保护，突变体建议 适当接种疫苗的人不会有感染 S基因突变病毒。其次，发现了一个双向启动子复合体。 在HBVX基因内滴定，进一步强调了复杂的基因 这种病毒的组织。