MOLECULAR AND COMPUTER ANALYSIS OF THE HEPATITIS B VIRUS GENOME

乙型肝炎病毒基因组的分子和计算机分析

• 批准号: 3809699
• 负责人: R H MILLER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809699
• 关键词: biochemical evolution; computer assisted sequence analysis; duck hepatitis B virus; genetic enhancer element; genetic promoter element; genome; hepatitis B; hepatitis B virus group; nucleic acid hybridization; nucleic acid sequence; open reading frames; transcription factor; virus DNA; virus genetics

The purpose of this project is to analyze the HBV genome using computer and molecular techniques. Our computer analysis reveals several important findings: (1) We made a detailed study of the organization of the HBV genome and find that it is extremely compact because the virus encodes proteins from overlapping translation frames and because all cis-acting control elements are located within protein encoding sequences. (2) We find that the avian hepadnaviruses possess sequences within the central domain of the core gene that are similar to mammalian hepadnavirus X gene sequences. (3) HBV and retroviruses share features of genome organization and replication. This is significant because it suggests that these diverse virus families evolved from a common ancestral virus.

本项目的目的是利用计算机分析HBV基因组， 分子技术我们的计算机分析揭示了几个重要的 结果：（1）对HBV的组织结构进行了详细的研究 基因组，并发现它是非常紧凑的，因为病毒编码 蛋白质从重叠的翻译框架，因为所有的顺式作用 控制元件位于蛋白质编码序列内。(2)我们发现 禽嗜肝DNA病毒具有位于 与哺乳动物嗜肝DNA病毒X基因相似的核心基因 序列的(3)HBV和逆转录病毒具有相同的基因组结构特征 和复制。这一点很重要，因为它表明，这些不同的 病毒家族是从共同的祖先病毒进化而来的。