ACETOGENIC AUTOTROPHY AND METABOLISM OF NICKEL

镍的乙酸自养和代谢

• 批准号: 3070795
• 负责人: HAROLD L DRAKE
• 金额: $ 5.12万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3070795
• 关键词: Clostridium; bioenergetics; carbon; enteric bacteria; enzyme mechanism; metal metabolism; metalloproteins; microorganism culture; microorganism growth; microorganism metabolism; nickel; oxidoreductase; protein structure; radiotracer

Nickel is a biologically active trace metal important to mammalian health, as are numerous obligately anaerobic bacteria which inhabit the microintestinal environment. This study centers on further resolving (i) the biochemical/biological nature of nickel in acetogenic bacteria and (ii) the autotrophic metabolism of this biological group. Specifically, the biochemistry and physiological function of the nickel enzyme carbon monoxide dehydrogenase will be further resolved and acetogenic hydrogenase will be purified and characterized. The regulation and intracellular localization of autotrophic enzymes will be studied in effort to gain insights into their physiological roles. Antibodies (rabbit) against key catalysts may be used as specific probes to aid in this elucidation. 14C tracer and product profile analyses will be conducted to study whole cell carbon flow and energetics of autotrophic vs. heterotrophic growth. Additionally, studies on recently discovered nickel proteins will be initiated to ascertain their potential physiological functions. Defined minimal media developed for the cultivation of acetogenic bacteria will facilitate studies on acetogenic nickel transport and trace metal requirements. Since acetogens and most of their enzymes are extremely sensitive to oxidation, all cultivation, purification, and analytical procedures will be conducted under strict anaerobiosis.

镍是一种具有生物活性的痕量金属，对哺乳动物的健康非常重要， 就像许多必需的厌氧细菌一样 微肠道环境。本研究以进一步解决问题为中心(一) 产乙酸菌中镍的生化/生物学性质和(Ii) 该生物群的自养新陈代谢。具体地说， 镍酶碳的生物化学和生理功能 一氧化碳脱氢酶将进一步分解和乙酰化氢酶 将被提纯和鉴定。调控与细胞内 将研究自养酶的本地化，以努力获得 对他们生理角色的洞察。KEY抗体(兔) 可以使用催化剂作为特定的探针来帮助这一阐明。14C 将进行示踪剂和产品图谱分析，以研究整个细胞 自养与异养生长的碳流和能量学。 此外，对最近发现的镍蛋白的研究将 启动以确定其潜在的生理功能。已定义 为培养产乙酸菌而开发的最低限度的培养基将 促进乙酰化镍迁移和痕量金属的研究 要求。因为乙酸菌和它们的大多数酶都是 对氧化敏感，所有培养、纯化和分析 手术将在严格的厌氧条件下进行。