NICKEL AND ACETOGENIC AUTOTROPHIC METABOLISM

镍与乙酸自养代谢

• 批准号: 3132269
• 负责人: HAROLD L DRAKE
• 金额: $ 7.48万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3132269
• 关键词: Clostridium; bacterial genetics; bacterial proteins; enzyme mechanism; metal metabolism; microorganism metabolism; nickel; physiology; trace elements

Nickel is a biologically active trace metal important to mammalian health, as are numerous obligately anaerobic bacteria which inhabit the microintestinal environment. The proposed study centers on further resolving i) the biochemical/biological nature of nickel in acetogenic bacteria, and ii) the autotrophic metabolism of this biological group. Specifically, we plan to continue our studies on the biochemistry and physiological function of the nickel enzyme, carbon monoxide dehydrogenase, and to characterize acetogenic hydrogenase. Further analyses on the regulation of these enzymes will yield insights into their physiological roles, and antibodies (rabbit) against these catalysts will be used as specific probes to aid in this elucidation. Additionally, purification of recently discovered metallo nickel proteins (currently of unknown function) will be undertaken, as will studies on nickel transport and the genetics of acetogenic bacteria. A recently developed minimally defined medium will facilitate this latter work. Since acetogens are obligate anaerobes and are sensitive to oxidation, anaerobic cultivation and purification procedures will be utilized throughout the investigation.

镍是一种具有生物活性的微量金属，对哺乳动物的健康很重要，