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NICKEL AND ACETOGENIC AUTOTROPHIC METABOLISM

镍与乙酸自养代谢

基本信息

批准号：
3132266
负责人：
HAROLD L DRAKE
金额：
$ 14.45万
依托单位：
UNIVERSITY OF MISSISSIPPI
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-12-01 至 1991-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3132266
关键词：
Clostridium Streptococcus bacterial genetics bacterial proteins enzyme mechanism high performance liquid chromatography metal metabolism microorganism metabolism nickel trace elements

项目摘要

Significance: Acetogenic bacteria play important roles in the flow of carbon and energy in anaerobic habitats, including the human gastrointestinal tract. The unique autotrophic pathway used by acetogens is termed the acetyl-CoA (or Wood) pathway, and studies have shown that acetogenesis is a fundamental biological process. New and diverse metabolic activities have recently been elucidated for acetogens, including the capacity to derive both carbon and energy from aromatic compounds. These potentials may contribute to the survival and interaction of acetogens in their native habitats. In general, the anaerobic metabolism of aromatic compounds is poorly defined, and the acetogenic metabolism of such substrates is likely coupled to the overall biotransformation of lignin and other aromatic substrates, including aromatic toxic wastes. Abstract of Research Plan: Strictly defined environments for resolution of the metabolic potentials of acetogenic bacteria have been developed, and new enzyme systems central to acetogenesis from aromatic compounds have been discovered. The main goal of this project will be to further resolve these newly realized catalytic activities. The classic acetogen Clostridium thermoaceticum, the human gastrointestinal isolate Peptostreptococcus productus, and other acetogens will be used in this study. Methods will include: enzyme analyses on the flow of carbon and reductant from aromatic compounds; 13C and 14C tracer analyses; high performance liquid chromatographic (HPLC) analyses; high-resolution fast protein liquid chromatography (FPLC) for the purification of enzyme systems; and the use of enzyme-specific antibodies as molecular probes and inhibitors. The specific aims include: (i) Resolving the specificity and diversity of new capacities observed for acetogenesis from aromatic compounds. (ii) Elucidating how carbon and reductant which are derived from aromatic substituent groups are utilized in the acetyl-CoA pathway. (iii) The purification and characterization of new enzyme systems involved in these processes.

意义：产乙酸菌在生物降解过程中起着重要的作用，包括人类在内的厌氧栖息地的碳和能源胃肠道产乙酸菌独特的自养途径被称为乙酰辅酶A（或伍德）途径，研究表明，产乙酸是基本的生物过程。新的和多样化最近已经阐明了产乙酸菌的代谢活性，包括从芳香族化合物中获得碳和能量的能力，化合物. 这些潜力可能有助于生存，产乙酸菌在其原生生境中的相互作用。总体上芳香族化合物的厌氧代谢定义不明确，这种底物的产乙酸代谢可能与总的木质素和其它芳香底物的生物转化，包括芳香有毒废物。研究计划摘要：严格定义的解决环境产乙酸细菌的代谢潜力已经被开发，和新的酶系统从芳香族化合物中合成乙酸被发现了该项目的主要目标是进一步解决这些新实现的催化活动。典型的产乙酸菌热醋酸梭菌人胃肠分离株 Peptostreenchproductus和其他产乙酸菌将用于本发明。 study. 方法将包括：对碳流的酶分析，芳香族化合物还原剂; 13C和14C示踪分析;高高效液相色谱（HPLC）分析;高分辨率快速蛋白质液相色谱法（FPLC）用于酶的纯化系统;以及使用酶特异性抗体作为分子探针，抑制剂的具体目标包括： (i)解决观察到的新能力的特殊性和多样性为从芳香族化合物中合成乙酸。 (ii)阐明了芳烃中碳和还原剂的生成在乙酰辅酶A途径中利用取代基。 (iii)新酶系的纯化和性质研究在这些过程中。