GENE SPECIFIC DNA REPAIR

基因特异性 DNA 修复

• 批准号: 5200341
• 负责人: V A BOHR
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5200341
• 关键词: DNA damage; DNA repair; antisense nucleic acid; chromosome translocation; enzyme inhibitors; fluorescence microscopy; gene rearrangement; genetic regulation; genetic transcription; mutant; transfection

We are studying the molecular biochemistry and fine structure of DNA repair. This includes work on the regulation and the mechanism of gene specific repair and transcription-repair coupling. We are studying DNA repair coupling with transcription with a view to clarify which gene products are involved and how these processes are regulated as compared to the DNA repair processes in the general, overall bulk of the genome. We use different approaches to the study of the regulation of gene specific DNA repair. They include the use of specific enzyme inhibitors, antisense systems, mutant cell lines, cell lines transfected with specific repair genes, fluorescence microscopy, and in vitro DNA repair assays. There are distinct differences in the efficiency of gene- and strand specific DNA repair dependent upon the type of DNA damage, and it is possible that the local degree of chromosomal distortion is the important element in determining the repair response chosen by the cell. We are suggesting that proficient DNA repair is necessary to secure genomic stability, and we find that certain regions of the genome that undergo translocation or rearrangements are poorly repaired.

我们正在研究DNA的分子生物化学和精细结构 修复.这包括对基因的调控和机制的研究， 特异性修复和转录修复偶联。 我们在研究DNA 修复与转录的偶联，以澄清哪个基因 以及这些过程是如何被监管的， 基因组整体的DNA修复过程。 我们用不同的方法来研究基因的调控， 特异性DNA修复 它们包括使用特定的酶抑制剂， 反义系统，突变细胞系，转染 特异性修复基因、荧光显微镜和体外DNA修复 测定。 在基因的效率上有明显的差异， 链特异性DNA修复取决于DNA损伤的类型，并且它 染色体畸变的局部程度可能是 这是决定细胞选择的修复反应的重要因素。 我们认为，熟练的DNA修复是必要的，以确保 基因组的稳定性，我们发现基因组的某些区域， 发生易位或重排的细胞修复不良。