DISCOVERY OF NOVEL HUMAN GENES BY AUTOMATED SEQUENCING OF CDNA LIBRARIES

在与L博士的持续合作中，国家癌症研究所的施陶德 通过自动化DNA发现新的人类淋巴特异性基因 消减cDNA文库的测序。DCRT开发的软件工具 用于处理数据并将其放入SYBASE关系数据库中 系统这些工具包括针对局部的cDNA序列进行预筛选的工具， 数据库，自动搜索上的非冗余数据库 NCBI网络BLAST服务器，提供结果的显示，并允许 用户交互选择要放入SYBASE的信息 数据库 正在开展工作，提供软件，以执行复杂的主题模式 匹配分析，例如搜索核定位信号， cDNA序列。该软件基于Genobase及其相关的 工具包，将允许结果自动纳入SYBASE 数据库，带有用于输入和编辑搜索的图形用户界面 参数 迄今为止，已经分析了数千个cDNA序列， 与多种蛋白质同源，包括转录调节因子， 信号转导蛋白和膜受体。工作正在进行 扩大数据库的范围，以包括实验室管理 来自其他来源的信息和数据，例如北方印迹。

In an ongoing collaboration with Dr. L. Staudt, NCI, an attempt is being made to discover novel human lymphoid-specific genes by automated DNA sequencing of subtracted cDNA libraries. Software tools developed by DCRT are used to process and place the data into a SYBASE relational database system. These include tools for prescreening cDNA sequence against a local database, automated searching against the nonredundant databases on the NCBI network BLAST server, providing display of the results, and allowing user interaction to select information to be placed into the SYBASE database. Work is under way to provide software to perform complex motif pattern matching analyses, such as searches for nuclear localization signals, on the cDNA sequences. This software, based on Genobase and its associated toolkit, will permit automated incorporation of results into the SYBASE database, with a graphical user interface for input and editing of search parameters. To date, thousands of cDNA sequences have been analyzed, yielding homologies to a variety of proteins, including transcriptional regulators, signal transduction proteins and membrane receptors. Work is in progress to expand the scope of the database to include laboratory management information and data from other sources, such as northern blots.