COMPUTATIONAL RESOURCES FOR AUTOMATED DNA SEQUENCING OF SUBTRACTED CDNA LIBARIES

与美国国立卫生研究院L.Staudt博士合作发现新人 消减文库DNA自动测序技术研究淋系特异性基因 图书馆。DCRT开发的软件工具用于处理和 将数据放入Sybase关系数据库系统。这些措施包括 根据本地数据库预先筛选cdna序列的工具，自动化 NCBI网络BLAST上的非冗余数据库检索 服务器，并提供结果的显示，允许用户 用于选择要放入Sybase数据库的信息的交互。 开始提供软件来执行复杂的图案图案 对获得的DNA序列进行比对分析。 到目前为止，已经分析了大约1500个cdna序列，产生了 与多种蛋白质的同源性，包括转录调节因子， 信号转导蛋白和膜受体。工作正在进行中 扩大数据库的使用范围，将实验室管理包括在内 来自其他来源的信息和数据，如北方的地块。

A collaboration with Dr. L. Staudt, NCI, on the discovery of novel human lymphoid-specific genes by automated DNA sequencing of subtracted cDNA libraries. Software tools developed by DCRT are used to process and place the data into a SYBASE relational database system. These include tools for prescreening cDNA sequence against a local database, automating searching against the non-redundant databases on the NCBI network BLAST server, and providing for the display of the results, allowing user interaction to select information to be placed into the SYBASE database. Work was initiated to provide software to perform complex motif pattern matching analysis on the cDNA sequences. To date, approximately 1,500 cDNA sequences have been analyzed, yielding homologies to a variety of proteins including transcriptional regulators, signal transduction proteins and membrane receptors. Work is in progress to expand the use of the database to include laboratory management information and data from other sources such as northern plots.