COMPUTATIONAL RESOURCES FOR AUTOMATED DNA SEQUENCING OF SUBTRACTED CDNA LIBARIES

用于扣除 CDNA 文库自动 DNA 测序的计算资源

• 批准号: 3774983
• 负责人: J I POWELL
• 金额: --
• 依托单位: CENTER FOR INFORMATION TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774983
• 关键词: DNA; biological signal transduction; biomedical automation; complementary DNA; computer assisted sequence analysis; computer graphics /printing; computer human interaction; computer program /software; genetic library; mathematical model; northern blottings; nucleic acid sequence; receptor; transcription factor

A collaboration with Dr. L. Staudt, NCI, on the discovery of novel human lymphoid-specific genes by automated DNA sequencing of subtracted cDNA libraries. Software tools developed by DCRT are used to process and place the data into a SYBASE relational database system. These include tools for prescreening cDNA sequence against a local database, automating searching against the non-redundant databases on the NCBI network BLAST server, and providing for the display of the results, allowing user interaction to select information to be placed into the SYBASE database. Work was initiated to provide software to perform complex motif pattern matching analysis on the cDNA sequences. To date, approximately 1,500 cDNA sequences have been analyzed, yielding homologies to a variety of proteins including transcriptional regulators, signal transduction proteins and membrane receptors. Work is in progress to expand the use of the database to include laboratory management information and data from other sources such as northern plots.

与L博士合作。施陶德，国家癌症研究所，关于发现新的人类 通过消减cDNA的自动DNA测序法检测淋巴特异性基因 图书馆. DCRT开发的软件工具用于处理和 将数据放入SYBASE关系数据库系统中。 这些包括 用于根据本地数据库预筛选cDNA序列的工具， 检索NCBI网络BLAST上的非冗余数据库 服务器，并提供结果的显示，允许用户 交互以选择要放入SYBASE数据库的信息。 工作开始提供软件来执行复杂的主题模式 cDNA序列的匹配分析。 迄今为止，已经分析了大约1,500个cDNA序列， 与包括转录调节因子在内的多种蛋白质具有同源性， 信号转导蛋白和膜受体。 工作正在进行 扩大数据库的使用范围，以包括实验室管理 其他来源的信息和数据，如北方地块。

项目成果

Galactosaemia with fatal cerebral oedema.

半乳糖血症伴有致命性脑水肿。

• DOI: 10.1007/bf01802050
• 发表时间: 1989
• 期刊: Journal of inherited metabolic disease
• 影响因子: 4.2
• 作者: Perelmuter,B;Goodman,SI;McCabe,ER
• 通讯作者: McCabe,ER