COMPUTER SUPPORT FOR MOLECULAR SEQUENCING AND GENETIC MAPPING

分子测序和遗传图谱的计算机支持

• 批准号: 3838531
• 负责人: J I POWELL
• 金额: --
• 依托单位: CENTER FOR INFORMATION TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838531
• 关键词: computer assisted sequence analysis; computer center; computer data analysis; computer program /software; genetic mapping; genome; human genetic material tag; information retrieval; information system analysis; parallel processing

Computer Systems Laboratory (CSL) has integrated laboratory acquisition and data processing computer systems in the Receptor Biochemistry and Molecular Biology Branch (RBMB), NINDS. Seven automated DNA sequencers produce 168 sequence fragments or about 60,000 bases of sequence per day. We have specified, installed and administered the computer facilities used for analyzing and archiving this large volume of DNA sequence data. In FY92 CSL provided system management, programming and database support for the cDNA Project, funded in part by the Department of Energy Center for Disease Control, that seeks to identify and characterize the nearly 30,000 genes expressed in the human brain, producing expressed sequence tags (ESTs) from human brain cDNAs at the rate of approximately 100 templates/day. Over 6,000 EST sequences have been published and submitted to GenBank. Similarly, the goal of the smallpox project, a collaboration with the Department of Energy Centers for Disease Control (DECDC), is to sequence completely the 170 kilo-base smallpox genome prior to its destruction in December, 1993. Genome sequencing and assembly is nearly complete and analysis of the finished sequence is underway. System management assistance included daily operations support for this high-volume production system, specification and initiation of system backup procedures, and installation and testing of commercial and public domain software. A set of programs was created to batch process ESTs for database searching. A protein library motif comparison program was ported to the CSL parallel computer. CSL is purchasing a special purpose sequence assembly and analysis system based on a high speed text search computational engine to be made available to the NIH intramural community as a shared resource connected to the NIH network. During FY92, emphasis has continued to shift from sequence assembly to sequence analysis and data archival/retrieval. CSL will continue to concentrate on methods for processing and archiving extremely large volumes of sequence data, and help to make technologies developed through this project available to other investigators at NIH.

计算机系统实验室(CSL)将实验室采购和 受体生物化学与分子研究中的数据处理计算机系统 NINDS生物学分会(RBMB)。七台自动DNA测序仪产生168个 序列碎片或每天大约60,000个碱基的序列。我们有 指定、安装和管理下列计算机设施 分析和归档这些海量的DNA序列数据。 在92财年，CSL提供了系统管理、编程和数据库支持 对于cDNA项目，部分资金来自能源部中心 疾病控制，旨在确定和描述近30,000人的特征 在人脑中表达的基因，产生表达的序列标签 (EST)以大约100%的速度从人脑cDNA中分离出来 模板/天。目前已发表和提交了6000多个EST序列 致GenBank。同样，天花项目的目标是一项合作 与能源部疾病控制中心(DECDC)合作，是为了 在ITS之前对170 kb的天花基因组进行完全测序 1993年12月的破坏。基因组测序和组装几乎是 对完成的序列的完成和分析正在进行中。 系统管理协助包括对此的日常运营支持 大批量生产体系、体系规范和体系启动 备份程序以及商业和公共设备的安装和测试 域软件。创建了一组程序来批处理EST 数据库搜索。移植了蛋白质文库基序比对程序 到CSL并行计算机。 CSL正在购买一种特殊用途的序列组装和分析系统 基于高速文本搜索计算引擎将可用 作为与NIH连接的共享资源提供给NIH内部社区 网络。 在92财年，重点继续从序列组装转移到 序列分析和数据存档/检索。中超将继续 专注于处理和归档超大容量卷的方法 序列数据，并帮助通过这种方式开发的技术 该项目可供NIH的其他调查人员使用。