DISCOVERY OF NOVEL HUMAN GENES BY AUTOMATED SEQUENCING OF CDNA LIBRARIES

正在与L博士合作。施陶德，国家癌症研究所， 通过自动DNA测序获得新的人类类人猿特异性基因， 消减cDNA文库。 DCRT开发的软件工具用于 处理并将数据放入SYBAS关系数据库系统。 这些工具包括针对局部的cDNA序列进行预筛选的工具， 数据库，自动搜索 NCBI网络BLAST服务器，提供结果的显示 允许用户交互以选择要放置到 SYBASE数据库。 迄今为止，已经分析了大约1500个cDNA序列， 与包括转录调节因子在内的多种蛋白质具有同源性， 信号转导蛋白和膜受体。 努力开发和完善自动化主题模式的方法 匹配和数据库分析，并纳入实验室管理 来自其他来源的信息和数据，如北方印迹， SYBASE数据库继续。

A collaboration is ongoing with Dr. L. Staudt, NCI, on the discovery of novel human Lymphoid-specific genes by automated DNA sequencing of subtracted cDNA libraries. Software tools developed by DCRT are used to process and place the data into a SYBAS relational database system. These include tools for prescreening cDNA sequence against a local database, automating searching against the non-redundant databases on the NCBI network BLAST server, providing for the display of the results allowing user interaction to select information to be placed into the SYBASE database. To date, approximately 1500 cDNA sequences have been analyzed yielding homologies to a variety of proteins including transcriptional regulators, signal transduction proteins and membrane receptors. Efforts to develop and refine methods for automating motif pattern matching and database analysis, and to incorporate laboratory management information and data from other sources such as northern blots into the SYBASE database continue.