B19 PARVOVIRUS AND ADENO-ASSOCIATED VIRUS

我们的实验室对B19细小病毒进行基础和临床研究， 细小病毒科中在人类身上致病的唯一成员。急性 感染导致第五种疾病，儿童皮疹疾病和 成人多关节痛综合征。在患有潜在疾病的患者中 溶血、急性感染导致一过性再生障碍性危象。在……里面 有潜在免疫缺陷的患者，病毒感染持续存在 导致慢性贫血；细小病毒感染是导致贫血的原因之一 艾滋病患者。该病毒对红系祖细胞具有亲和性。 由于其使用的是红细胞P抗原。在过去的一年里，我们已经 发现了两种新的细小病毒病。宫内感染可导致 患有先天性贫血症。在一些儿童中，细小病毒感染可能是 随后是一种称为阵发性感冒的综合征中的严重溶血性贫血 血红蛋白尿。在疫苗研究的继续中，我们已经证明 这种合成肽由20个氨基酸组成，源自独特的区域 在小衣壳蛋白中，诱导中和抗体反应 兔子。B19细小病毒的遗传调控已阐明 通过鉴定具有功能活性的YY1结合位点 末端重复序列和启动子区域。一种有效的方法 在杆状病毒系统中对YY1进行了纯化。这个 非结构蛋白的细胞毒性作用已被证明依赖于 在完整的核苷酸三磷酸结合区。我们已经扩大了规模 微小病毒研究将腺相关病毒作为基因载体 心理治疗。我们已经证明了腺相关病毒可以转导 高表达人珠蛋白基因导入人造血祖细胞 效率，从而产生大量的蛋白质生产。一种方法，用于 腺相关病毒在细胞中的瞬时表达研究 已开发了选择性条件。

Our laboratory performs basic and clinical studies of the B19 parvovirus, the only member of the Parvoviridae family pathogenic in humans. Acute infection causes fifth disease, ad childhood rash illness and a polyarthralgia syndrome in adults. In patients with underlying hemolysis, acute infection results in transient aplastic crisis. In patients with underlying immunodeficiency, virus infection persists and causes chronic anemia; parvovirus infection is a cause of anemia in patients with AIDS. The virus is tropic for erythroid progenitor cells due to its use os erythrocyte P antigen. during the past year, we have identified two new parvovirus diseases. In utero infection can result in congenital anemia. In some children, parvovirus infection can be followed by severe hemolytic anemia in a syndrome termed paroxysmal cold hemoglobinuria. In continuation of vaccine studies, we have demonstrated that synthetic peptides of 20 amino acids, derived from the unique region of the minor capsid protein, elicit neutralizing antibody responses in rabbits. The genetic regulation of B19 parvovirus has been elucidated by identification of functionally active YY1 binding sites in the terminal repeat and promoter regions. An efficient method of purification of YY1 in a baculovirus system has been developed. The cytotoxic effect of the nonstructural protein has been shown to depend on intact nucleotide triphosphate binding regions. We have expanded out parvovirus studies to include adeno-associated virus as a vector for gene therapy. We have demonstrated that adeno-associated virus can transduce a human globin gene into human hematopoietic progenitor cells with high efficiency, resulting in significant protein production. A method to study transient expression of adeno-associated virus in cells under non- selective conditions has been developed.