PAROVIRUS

细小病毒

• 批准号: 3942851
• 负责人: N S YOUNG
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3942851
• 关键词: Parvoviridae; Retroviridae; Retroviridae disease; aplastic anemia; feline leukemia /sarcoma virus; human tissue; tissue /cell culture; virus cytopathogenic effect; virus diseases

Studies of the B19 parvovirus have utilized a suspension culture system for propogration of the virus for detailed molecular analysis of virus replication, transcription, and protein synthesis. B19 parvovirus DNA replication is similar to that of other parvoviruses, proceeding through double-stranded high molecular weight intermediate forms. B19 parvovirus proteins are also similar to those of other parvoviruses, consisting of two capsid proteins of 58 and 83 kd and at least one nonstructural protein of 77 kd. In contrast to other parvoviruses, the transcription map of the B19 parvovirus is extraordinarily complex, and detailed analysis has shown that regulation, independent of promoter strength, likely results from differential splicing or transcription termination. Specific virus protein products have been assigned to RNA species, and viral proteins translated in vitro. A cytotoxic function has been assigned to the nonstructural protein using transfection of recombinant constructs into HeLa cells. In clinical studies, the spectrum of parvovirus disease has been expanded by the description of the first case of persistent B19 infection, in a child with congenital immunodeficiency, result in chronic bone marrow failure. The normal host defense to B19 parvovirus infection appears to be predominantly humoral and mediated by neutralizing antibodies. B19 parvovirus has been detected in the circulating cells of patients acutely infected and may be present in granulocyte precursors. In parallel studies of feline panleukopenia virus, a common natural cause of neutropenia and aplastic anemia in cats, virus replication and propagation have been detected in vitro using bone marrow cultures and shown to be dependent upon the addition of hematopoietic colony stimulating factors. Feline panleukopenia virus is a potent inhibitor of colony formation by cat progenitor cells from all lineages.

B19 细小病毒的研究利用了悬浮培养 病毒繁殖系统的详细分子信息 分析病毒复制、转录和蛋白质合成。 B19 细小病毒 DNA 复制与其他细小病毒相似 细小病毒，通过双链高分子进行 重量中间形式。 B19 细小病毒蛋白也 与其他细小病毒相似，由两个衣壳组成 58 和 83 kd 的蛋白质以及至少一种非结构蛋白质 77 公里 与其他细小病毒相比，转录图谱 B19 细小病毒极其复杂且详细 分析表明，监管独立于发起人 强度，可能是差异剪接或转录的结果 终止。 已指定特定病毒蛋白产品 RNA种类和病毒蛋白在体外翻译。 一个 细胞毒功能已被指定给非结构蛋白 使用重组构建体转染 HeLa 细胞。 在 临床研究表明，细小病毒病的谱 通过对第一例持久性 B19 的描述进行了扩展 先天性免疫缺陷儿童的感染会导致 慢性骨髓衰竭。 正常宿主对 B19 的防御 细小病毒感染似乎主要是体液感染 由中和抗体介导。 B19细小病毒已 在急性感染患者的循环细胞中检测到 可能存在于粒细胞前体中。 在并行研究中 猫泛白细胞减少症病毒，一种常见的自然原因 猫的中性粒细胞减少症和再生障碍性贫血、病毒复制和 已使用骨髓在体外检测到繁殖 文化并显示出依赖于添加 造血集落刺激因子。 猫泛白细胞减少症 病毒是猫祖细胞集落形成的有效抑制剂 来自所有谱系的细胞。