PAROVIRUS (HUMAN) B19

我们的实验室对B19细小病毒进行基础和临床研究， 人类致病的细小病毒科中唯一的成员。急性 感染导致第五种疾病，一种儿童皮疹疾病和一种 成人多关节痛综合征。在患有潜在疾病的患者中 溶血、急性感染导致一过性再生障碍性危象。在……里面 有潜在免疫缺陷的患者，病毒感染持续存在 导致慢性贫血；细小病毒感染是导致贫血的原因之一 艾滋病患者。该病毒对红系祖细胞有营养作用。 我们在B19细小病毒生物学方面取得了一些进展 最后一年。我们发现了首例先天性B19 细小病毒感染：病毒传播到胎儿，导致 出生时的慢性贫血和7个月时的死亡；病毒仅限于 骨髓并没有循环。一例成年患者的康复情况 先天性纯红细胞再生障碍性贫血，骨髓显示B19 提示白介素3可能诱导抗体形成。 这种病毒可能用于艾滋病相关感染的患者。 我们已经诱导了针对B19细小病毒的中和抗体 杆状病毒在动物体内产生空衣壳。这个空洞的衣壳系统 可以被操纵来增加次要衣壳蛋白的比例 次要衣壳蛋白抗原包含中和表位，因为 病毒组装、替代异源病毒不需要 小衣壳抗原独特区域的蛋白质序列构成 空的细小病毒衣壳可用作疫苗制剂和特异性 蛋白质的输送。我们已经实现了B19细小病毒的繁殖 在骨髓外使用来自一种 白血病患者。细小病毒对红系细胞的营养作用 已经在分子水平上进行了研究。基于以下模式 转染细小病毒序列的细胞中的RNA转录， P(6)启动子在非允许细胞中的功能和缺失 通过RNA保护分析突变，我们得出的结论是 基因组中间的转录存在于不允许的细胞中， 可能位于非功能p44下游的衰减器区域 推动者。

Our laboratory performs basic and clinical studies of the B19 parvovirus, the only member of parvoviradae family pathogenic in humans. Acute infection causes fifth disease, a childhood rash illness and a polyarthralgia syndrome in adults. In patients with underlying hemolysis, acute infection results in transient aplastic crisis. In patients with underlying immunodeficiency, virus infection persists and causes chronic anemia; parvovirus infection is a cause of anemia in patients with AIDS. The virus is trophic for erythroid progenitor cells. We have made progress in B19 parvovirus biology in a number of areas over the last year. We have discovered the first case of congenital B19 parvovirus infection: transmission of the virus to the fetus resulted in chronic anemia at birth and death at 7 months; virus was restricted to the marrow and did not circulate. The recovery of an adult patient with congenital pure red cell aplasia, whose bone marrow showed B19 parvovirus, suggests that interleukin-3 may induce antibody formation to this virus, possibly of use in patients with AIDS-associated infection. We have induced neutralizing antibodies to B19 parvovirus using baculovirus-generated empty capsids in animals. This empty capsid system can be manipulated to increase the proportion of the minor capsid protein the minor capsid protein antigen contains a neutralizing epitope Because it is not required for virus assembly, substitution of heterologous proteins sequences in the unique region of the minor capsid antigen make empty parvovirus capsids useful as vaccine agents and for specific delivery of proteins. we have achieved propagation of B19 parvovirus outside of bone marrow using a megakaryocyte cell line derived from a patient with leukemia. The trophism of parvovirus for erythroid cells has been investigated at the molecular level. Based on the pattern of RNA transcription in cells transfected with parvovirus sequences, the function of the p(6) promotor in nonpermissive cells, and deletion mutations analyzed by RNA protection, we have concluded that a block in transcription in the middle of the genome exists in nonpermissive cells, possibly at an attenuator region downstream from the nonfunctional P44 promoter.