IDENTIFICATION AND CHARACTERIZATION OF A NOVAL PEPTIDE OSTEOCLASTPOIETIC FACTOR

新型肽破骨细胞生成因子的鉴定和表征

• 批准号: 3770129
• 负责人: TOSHIYUKI YONEDA
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3770129
• 关键词: animal tissue; autoradiography; bioassay; bone metabolism disorder; chickens; cytokine; gene expression; genetic library; human subject; immunoaffinity chromatography; laboratory mouse; laboratory rat; molecular cloning; organ culture; osteoclast activating factor; osteoclasts; osteogenesis; pathologic bone resorption; physiologic bone resorption; polymerase chain reaction; protein purification; protein sequence

We have identified a novel factor which stimulates the formation of osteoclasts and which is produced by a human tumor as well as by leukocytes which have been stimulated with concanavalin A. We have called this peptide osteoclastpoietic factor (OPF). We have obtained amino acid sequence on this peptide and found that it is novel and distinct from other known growth regulatory factors, interleukins, and colony stimulating factors. We have found that a synthetic peptide which corresponds to this amino acid sequence mimics the biological effects, and we have produced both monoclonal and polyclonal antibodies which neutralize the original biological activity. In this project, we plan to: 1. Clone the gene and express the recombinant molecule. 2. Determine the role of OPF in normal bone remodeling and in disease states associated with increased bone resorption. Characterization of novel cytokines which are powerful stimulators of osteoclastic bone resorption is likely to lead to new insights in the normal control of osteoclast function and in the pathophysiology of disease states associated with bone destruction.

我们发现了一种新的因素，它能刺激……的形成