FACTORS INFLUENCING GENETIC TRANSCRIPTION--INITIATION AND TERMINATION

影响基因转录的因素——起始和终止

• 批准号: 3778508
• 负责人: R CROUCH
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3778508
• 关键词: DNA replication origin; Escherichia coli; RNA directed DNA polymerase; Retroviridae; bacterial genetics; enzyme mechanism; enzyme structure; gene expression; genetic manipulation; genetic transcription; molecular genetics; pancreatic ribonuclease; polymerization; protein sequence; protein structure function; transcription factor; transcription termination

The aim of this project is to examine the involvement of certain ribonucleases in cellular events. The scope of this project has evolved to include protein chemistry, enzymatic activity, overproduction of proteins and the consequences of changes in the level of enzymatic activity within the cell. In particular, ribonucleases specific for RNA of RNA-DNA hybrids are being examined for their functional role within the cell. Multiple enzymes exhibiting RNase H activity have been reported within cells and our experiments are aimed at understanding the need for multiple forms of the enzyme and what effects there would be upon inhibition of each (or all) of the RNase H activities. This is particularly relevant since the AIDS virus requires the functioning of its own RNase H to be infectious.

这个项目的目的是检查某些人的参与情况 细胞事件中的核糖核酸酶。这个项目的范围已经发生了变化 包括蛋白质化学，酶活性，过量生产 蛋白质和酶水平变化的后果 细胞内的活动。尤其是RNA特有的核糖核酸酶 的RNA-DNA杂交体正在接受检查，以确定它们在 手机。已有多种显示RNaseH活性的酶的报道 在细胞内，我们的实验旨在了解 这种酶的多种形式以及它们对 抑制每一个(或全部)核糖核酸酶H活性。这是 特别重要的是，因为艾滋病病毒需要其功能 拥有核糖核酸酶H具有传染性。