FACTORS INFLUENCING GENETIC TRANSCRIPTION--INITIATION AND TERMINATION

影响基因转录的因素——起始和终止

• 批准号: 5203275
• 负责人: R CROUCH
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203275
• 关键词: DNA gyrase; DNA topoisomerases; Escherichia coli; RNA directed DNA polymerase; Retroviridae; bacterial genetics; double stranded RNA; enzyme mechanism; enzyme structure; genetic transcription; nucleic acid hybridization; protein structure function; ribonuclease III; transcription termination

Ribonuclease H (RNase H) plays crucial roles in replication of retroviruses, including the human immunodeficiency virus (HIV) as well as involvement in normal cellular events including DNA replication. The purpose of this project is to gain a detailed understanding of the precise nature of the substrates for RNases H, the chemical makeup of cellular RNases H. The enzymes derived from cellular sources have higher specific activities than those of retroviral origin and have somewhat different specificities. We have found that separation of the RNase H domain of MuLV reverse transcriptase from the polymerase domain creates an active enzyme with specificities different from the parental RNase H but still with low specific activity. If we produce RNases H from yeasts, they have high specific activities regardless of their association with their own special domains. These results indicate that the differences in these proteins reside in the RNase H domain itself.

核糖核酸酶 H (RNase H) 在复制过程中发挥着至关重要的作用 逆转录病毒，包括人类免疫缺陷病毒（HIV） 参与正常细胞事件，包括 DNA 复制。 这 该项目的目的是详细了解 RNase H 底物的精确性质、化学组成 细胞核糖核酸酶 H. 细胞来源的酶具有更高的 比逆转录病毒起源的活性具有特定的活性，并且具有一定的 不同的具体情况。 我们发现 RNase H 的分离 MuLV逆转录酶的结构域从聚合酶结构域创建 一种活性酶，其特异性不同于亲本 RNase H 但比活性仍然较低。 如果我们生产 RNase H 酵母，无论其性质如何，它们都具有高比活性 与自己的特殊领域的联系。 这些结果表明 这些蛋白质的差异在于 RNase H 结构域本身。