FACTORS INFLUENCING GENETIC TRANSCRIPTION--INITIATION AND TERMINATION

影响基因转录的因素——起始和终止

• 批准号: 3842238
• 负责人: R CROUCH
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3842238
• 关键词: Escherichia coli; bacterial genetics; enzyme mechanism; enzyme structure; gene expression; genetic manipulation; genetic transcription; molecular genetics; pancreatic ribonuclease; protein sequence; protein structure function; transcription factor; transcription termination

The aim of this project is to examine the involvement of certain ribonucleases in cellular events. The scope of this project has evolved to include protein chemistry, enzymatic activity, overproduction of proteins and the consequences of changes in the level of enzymatic activity within the cell. The basis for most of the experimental approaches is derived from an enzymological perspective. The ability to examine a particular event in great detail from primary amino acid sequence of a protein to the consequences of alteration of the level of enzymatic activity within the cell has given us the opportunity to study both the enzymological and functional roles of these intriguing enzymes. A second area of interest is mutagenesis in E. coli.

这个项目的目的是检查某些人的参与情况 细胞事件中的核糖核酸酶。这个项目的范围已经发生了变化 包括蛋白质化学，酶活性，过量生产 蛋白质和酶水平变化的后果 细胞内的活动。大多数实验的基础 方法是从酶学的角度得出的。有能力 从初级氨基酸中详细地研究一个特定的事件 蛋白质序列对蛋白质水平变化的影响 细胞内的酶活性给了我们研究的机会 这些耐人寻味的酶的酶学和功能作用。 第二个令人感兴趣的领域是大肠杆菌中的突变。