FACTORS INFLUENCING GENETIC TRANSCRIPTION-- INITIATION AND TERMINATION

影响基因转录的因素——起始和终止

• 批准号: 3857049
• 负责人: R CROUCH
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3857049
• 关键词: Escherichia coli; bacterial genetics; enzyme mechanism; enzyme structure; gene expression; genetic manipulation; genetic transcription; molecular genetics; pancreatic ribonuclease; protein sequence; protein structure function; transcription factor; transcription termination

The aim of this project is to examine the involvement of certain ribonucleases in cellular events. The scope of this project has evolved to include protein chemistry, enzymatic activity, overproduction of proteins and the consequences of changes in the level of enzymatic activity within the cell. The basis for most of the experimental approaches is derived from an enzymological perspective. The ability to examine a particular event in great detail from primary amino acid sequence of a protein to the consequences of alteration of the level of enzymatic activity within the cell has given us the opportunity to study both the enzymological and functional roles of these intriguing enzymes. A second area of interest is mutagenesis in E. coli.

这个项目的目的是研究某些