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TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES

TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
CDNAS 转染药物代谢酶
批准号：
3755363
负责人：
R LANGENBACH
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3755363
关键词：
Retroviridae anthracenes antiinflammatory agents chemical carcinogen complementary DNA cytochrome P450 drug metabolism enzyme activity enzyme inhibitors gene mutation isozymes mutagen testing mutagens nitrosamines protein isoforms radioimmunoassay tissue /cell culture transfection transfection /expression vector western blottings

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英文摘要
Retroviral vectors have been used to transfer and express the cDNAs for drug metabolizing enzymes (cytochromes P450 2A6 and 4B1, FMO, and PHS-1 & PHS-2) into C3H10T1/2 and AS52 cells. C3H10T1/2 cells expressing the human cytochrome P450 2A6 were mutated and morphologically transformed by the tobacco specific nitrosamine, NNK. AS52 cells expressing 2A6 were mutated by NNK and the mutational spectra of this potential human carcinogen determined. C3H10T1/2 cells expressing rabbit cytochrome P450 4B1 were sensitive to the cytotoxic effects of ipomeanol and AF and have been shown to be mutated by ipomeanol. AS52 cells containing the rabbit lung FMO and mouse PHS-1 AND PHS-2 have recently been derived. Studies to determine if mutational spectra vary when a chemical is activated by different P450s or species dependent isoforms are in progress.
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TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
MOUSE MODEL--TARGETED GENE KNOCK OUT OF CYTOSOLIC PHOSPHOLIPASE A2
TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
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