TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES

逆转录病毒载体已被用于转移和表达cDNA。 药物代谢酶(细胞色素P450 2A6和4B1、FMO和 PHS-1和PHS-2)转化C3H10T1/2和AS52细胞。C3H10T1/2细胞 表达人细胞色素P450 2A6基因突变后 烟草特有的亚硝胺NNK对其进行了形态转化。 NNK诱变表达2A6的AS52细胞 确定了这种潜在的人类致癌物的光谱。C3H10T1/2细胞 表达兔细胞色素P4504B1对细胞毒敏感 异丙酚和房颤的作用，并已被证明是突变的 异丙肾上腺素。含兔肺FMO和小鼠PHS-1的AS52细胞 和PHS-2是最近衍生出来的。研究以确定是否 当一种化学物质被不同的P450激活时，突变光谱会有所不同 或物种依赖的异构体正在进行中。

Retroviral vectors have been used to transfer and express the cDNAs for drug metabolizing enzymes (cytochromes P450 2A6 and 4B1, FMO, and PHS-1 & PHS-2) into C3H10T1/2 and AS52 cells. C3H10T1/2 cells expressing the human cytochrome P450 2A6 were mutated and morphologically transformed by the tobacco specific nitrosamine, NNK. AS52 cells expressing 2A6 were mutated by NNK and the mutational spectra of this potential human carcinogen determined. C3H10T1/2 cells expressing rabbit cytochrome P450 4B1 were sensitive to the cytotoxic effects of ipomeanol and AF and have been shown to be mutated by ipomeanol. AS52 cells containing the rabbit lung FMO and mouse PHS-1 AND PHS-2 have recently been derived. Studies to determine if mutational spectra vary when a chemical is activated by different P450s or species dependent isoforms are in progress.