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TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES

TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
CDNAS 转染药物代谢酶
批准号：
5202108
负责人：
R LANGENBACH
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/5202108
关键词：
Retroviridae aflatoxins aniline dye antiinflammatory agents arachidonate complementary DNA drug metabolism enzyme activity gene expression genetic transduction isozymes methylhydrazines nitric oxide phorbols prostaglandin endoperoxide synthase tissue /cell culture transfection transfection /expression vector

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英文摘要
Retroviral vectors have been used to transfer and express cyclooxygenase (COX) 1and 2. 10T1/2 and AS52 cells that stably express high levels of retroviral vector transferred murine COX-1 or -2 were developed and characterized. The expressed COX-2 preferentially utilized specific endogenous AA (arachidonic acid) pools (i.e., TPA released but not Ca ionophore released AA). Furthermore, NO (nitric oxide) was shown to enhance both COX-1 and -2 activities. The TPA and NO findings may be relevant to the COX's hypothesized role in carcinogenesis and inflammation. Additionally, we showed that COX-1 and -2 metabolically activated 1,1-dimethylhydrazine, aflatoxin B1, and N-acetylbenzidine to mutagens. Furthermore, the cells were useful in identifying NSAIDs which were selective for COX-1 and COX-2.
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TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
MOUSE MODEL--TARGETED GENE KNOCK OUT OF CYTOSOLIC PHOSPHOLIPASE A2
MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
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