TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES

逆转录病毒载体已被用于转移和表达环氧合酶 (COX)1和2.10T1/2和AS52细胞稳定高水平表达 构建了转导COX-1或COX-2的逆转录病毒载体， 特色化的。表达的COX-2优先利用特异性 内源性AA(花生四烯酸)池(即释放TPA但不释放Ca 离子载体释放AA)。此外，NO(一氧化氮)被显示为 提高COX-1和COX-2活性。TPA和没有调查结果可能是 与COX在癌症发生和发展中的假设作用有关 发炎。此外，我们还证明了COX-1和COX-2在代谢上 活化的1，1-二甲基肼、黄曲霉毒素B1和N-乙酰联苯胺 诱变剂。此外，这些细胞在识别非类固醇抗炎药方面也很有用 对COX-1和COX-2有选择性。

Retroviral vectors have been used to transfer and express cyclooxygenase (COX) 1and 2. 10T1/2 and AS52 cells that stably express high levels of retroviral vector transferred murine COX-1 or -2 were developed and characterized. The expressed COX-2 preferentially utilized specific endogenous AA (arachidonic acid) pools (i.e., TPA released but not Ca ionophore released AA). Furthermore, NO (nitric oxide) was shown to enhance both COX-1 and -2 activities. The TPA and NO findings may be relevant to the COX's hypothesized role in carcinogenesis and inflammation. Additionally, we showed that COX-1 and -2 metabolically activated 1,1-dimethylhydrazine, aflatoxin B1, and N-acetylbenzidine to mutagens. Furthermore, the cells were useful in identifying NSAIDs which were selective for COX-1 and COX-2.