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MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II

MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
小鼠模型——前列腺素合成酶I和II的靶向基因敲除
批准号：
3755386
负责人：
R LANGENBACH
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3755386
关键词：
Alphaherpesvirinae alleles bacterial genetics carcinogenesis disease /disorder model drug resistance embryonic stem cell gene deletion mutation genetically modified animals hypertension laboratory mouse molecular cloning neomycin nucleic acid sequence prostaglandin endoperoxide synthase site directed mutagenesis southern blotting thymidine kinase transposon /insertion element virus genetics

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中文摘要
翻译
小鼠前列腺素合成酶I基因的部分克隆 共获得129条基因组DNA。靶向构建物使用 带有插入细菌neo基因的7.5kb的基因组克隆和 拖尾单纯疱疹病毒TK基因。这个构造是 电穿孔进入胚胎干细胞与G418/更昔洛韦耐药 克隆被分离出来。同源靶向的ES细胞通过 Southern和聚合酶链式反应分析。具有单一等位基因的靶向ES细胞 被破坏的PHS-1已经被注射到巴斯德囊内，并且 胚泡被植入。嵌合体的诞生，随后， 正在等待生殖系传播的动物。这些动物将被利用 目的：探讨PHS-1在肿瘤发生中的作用。
英文摘要
A partial clone of the prostaglandin synthase I gene from mouse strain 129 genomic DNA was obtained. A targeting construct was made using 7.5kb of the genomic clone with an inserted bacterial neo gene and trailing herpes simplex virus TK gene. The construct was electroporated into embryonic stem cells and G418/gangcyclovir resistant clones were isolated. Homologously targeted ES cells were identified by Southern and PCR analysis. The targeted ES cells with a single allele of the PHS-1 disrupted have been injected into bastocysts and the blastocysts implanted. The births of chimeric and, subsequently, germline transmitting animals are awaited. These animals will be utilized to determine the role of PHS-1 in carcinogenesis.
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MOUSE MODEL--TARGETED GENE KNOCK-OUT OF PROSTAGLANDIN SYNTHASE I AND II
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TRANSFECTION OF CDNAS FOR DRUG METABOLIZING ENZYMES
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