ORGAN AND SPECIES SPECIFICITIES OF CHEMICAL CARCINOGENS

一种研究人体组织代谢和致突变性的体外方法 化学致癌物的激活已经被开发出来了。刚刚与世隔绝， 来自人类和啮齿动物的完整细胞用于新陈代谢 激活。鼠伤寒沙门氏菌的复原性用于评估生物 代谢物的活性和代谢物的高效液相分析 指挥。过去一年的工作重点是开发一套系统 用于频繁获取人体组织。人类系统已经被开发出来 研究人与人之间的差异和差异 人和啮齿动物肝、肾和结肠细胞激活/ 芳香胺的代谢。新陈代谢、诱变激活和 个体间的差异已经被测量和显著的差异 在人类个体之间发现了这些组织。这两种产品的比较 人类组织到相应的啮齿动物组织的研究正在进行中。

An in vitro approach for studying human tissue metabolism and mutagenic activation of chemical carcinogens have been developed. Freshly isolated, intact cells from humans as well as rodents are used for metabolic activation. Reversion of S. typhimurium is used to assess biological activity of metabolites and HPLC analysis of metabolites have been conducted. Work during the past year has centered on developing a system for frequent human tissue acquisition. A human system has been developed to investigate differences between individual humans and differences between human and rodent liver, kidney and colon cell activation/ metabolism of aromatic amines. Metabolism, mutagenic activation and inter-individual variation has been measured and significant differences found betwen individual humans for these tissues. Comparisons of these human tissues to the respective rodent tissues are in progress.