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REGULATION OF GENE ACTIVITY

REGULATION OF GENE ACTIVITY
基因活性的调节
批准号：
3916302
负责人：
I PASTAN
金额：
$0.0万
依托单位：
DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3916302
关键词：
RNA Retroviridae chemical binding epidermal growth factor genetic manipulation genetic mapping genetic promoter element genetic transcription genetic translation growth factor receptors messenger RNA molecular cloning neoplastic transformation nucleic acid sequence oncogenes oncoproteins protein biosynthesis receptor expression tissue /cell culture

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中文摘要
翻译
表皮生长因子受体（EGFR）在许多人类肿瘤中过度表达 暗示它与某些癌症的发病机制有关。 测试 为了研究EGFR在癌症中的作用，一种编码EGFR的逆转录病毒被 构建并显示引起3 T3的EGF依赖性转化 细胞 为了研究EGFR基因的调控， 含有许多转录因子结合位点 包括六个SP1站点和三个其他因子的站点。 因子 ETF 1刺激EGFR转录，具有120千道尔顿的Mr， 并结合到-230至-250区域。 因子ETF 2刺激EGFR和 和SV 40转录并结合到-120至-145。 EGF是 显示出在KB细胞中提高EGFR RNA水平。 这种效果似乎 这是由于EGFR RNA半衰期的增加。
英文摘要
The EGF receptor (EGFR) is overexpressed in many human tumors implicating it in the pathogenesis of some cancers. To test the role of the EGFR in cancer, a retrovirus encoding the EGFR was constructed and shown to cause EGF dependent transformation of 3T3 cells. To study regulation of the EGFR gene, the promoter region was shown to contain many transcription factor binding sites including six Sp1 sites and sites for three other factors. Factor ETF1 stimulates EGFR transcription, has a Mr of 120 kilodaltons, and binds to region -230 to -250. Factor ETF2 stimulates both EGFR and SV40 transcription and binds to -120 to -145. EGF has been shown to raise EGFR RNA levels in KB cells. This effect appears to be due to an increase in EGFR RNA half life.
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会议论文
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
MONOCLONAL ANTIBODIES TO CANCER CELLS
REGULATION OF GENE ACTIVITY
REGULATIION OF CANCER CELL GROWTH AND BEHAVIOR