REGULATION OF GENE ACTIVITY

基因活性的调节

• 批准号: 3916302
• 负责人: I PASTAN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916302
• 关键词: RNA; Retroviridae; chemical binding; epidermal growth factor; genetic manipulation; genetic mapping; genetic promoter element; genetic transcription; genetic translation; growth factor receptors; messenger RNA; molecular cloning; neoplastic transformation; nucleic acid sequence; oncogenes; oncoproteins; protein biosynthesis; receptor expression; tissue /cell culture

The EGF receptor (EGFR) is overexpressed in many human tumors implicating it in the pathogenesis of some cancers. To test the role of the EGFR in cancer, a retrovirus encoding the EGFR was constructed and shown to cause EGF dependent transformation of 3T3 cells. To study regulation of the EGFR gene, the promoter region was shown to contain many transcription factor binding sites including six Sp1 sites and sites for three other factors. Factor ETF1 stimulates EGFR transcription, has a Mr of 120 kilodaltons, and binds to region -230 to -250. Factor ETF2 stimulates both EGFR and SV40 transcription and binds to -120 to -145. EGF has been shown to raise EGFR RNA levels in KB cells. This effect appears to be due to an increase in EGFR RNA half life.

EGF 受体 (EGFR) 在许多人类肿瘤中过度表达 表明它与某些癌症的发病机制有关。 测试 EGFR 在癌症中的作用，编码 EGFR 的逆转录病毒 构建并显示可引起 3T3 的 EGF 依赖性转化 细胞。 研究 EGFR 基因启动子区的调控 显示含有许多转录因子结合位点 包括六个 Sp1 站点和三个其他因素的站点。 因素 ETF1刺激EGFR转录，Mr为120千道尔顿， 并结合至-230至-250区域。 因子 ETF2 刺激 EGFR SV40 转录并结合-120 至-145。 EGF已 显示可提高 KB 细胞中 EGFR RNA 水平。 出现这个效果 这是由于 EGFR RNA 半衰期的延长。