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REGULATION OF GENE ACTIVITY

REGULATION OF GENE ACTIVITY
基因活性的调节
批准号：
3916302
负责人：
I PASTAN
金额：
$0.0万
依托单位：
DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3916302
关键词：
RNA Retroviridae chemical binding epidermal growth factor genetic manipulation genetic mapping genetic promoter element genetic transcription genetic translation growth factor receptors messenger RNA molecular cloning neoplastic transformation nucleic acid sequence oncogenes oncoproteins protein biosynthesis receptor expression tissue /cell culture

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中文摘要
翻译
表皮生长因子受体(EGFR)在许多人类肿瘤中过度表达 这表明它与某些癌症的发病机制有关。要测试 EGFR在癌症中的作用，编码EGFR的逆转录病毒是 构建并证明可导致3T3依赖于EGF的转化 细胞。研究EGFR基因启动子区域的调控 被证明含有许多转录因子结合位点 包括6个Sp1位点和3个其他因素的位点。因素 ETF1刺激EGFR转录，具有120千道尔顿的MR， 并结合到-230至-250区域。ETF2因子刺激两种表皮生长因子受体 和SV40转录并结合到-120到-145。EGF一直是 显示可提高KB细胞中的EGFR RNA水平。这种效果就会出现 这是由于EGFR RNA半衰期增加所致。
英文摘要
The EGF receptor (EGFR) is overexpressed in many human tumors implicating it in the pathogenesis of some cancers. To test the role of the EGFR in cancer, a retrovirus encoding the EGFR was constructed and shown to cause EGF dependent transformation of 3T3 cells. To study regulation of the EGFR gene, the promoter region was shown to contain many transcription factor binding sites including six Sp1 sites and sites for three other factors. Factor ETF1 stimulates EGFR transcription, has a Mr of 120 kilodaltons, and binds to region -230 to -250. Factor ETF2 stimulates both EGFR and SV40 transcription and binds to -120 to -145. EGF has been shown to raise EGFR RNA levels in KB cells. This effect appears to be due to an increase in EGFR RNA half life.
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GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS
MONOCLONAL ANTIBODIES TO CANCER CELLS
REGULATION OF GENE ACTIVITY
GENETIC ANALYSIS OF THE MULTIDRUG RESISTANCE PHENOTYPE IN TUMOR CELLS