REGULATION OF GENE ACTIVITY

基因活性的调节

• 批准号: 3962990
• 负责人: I PASTAN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3962990
• 关键词: RNA; Rous sarcoma virus; epidermal growth factor; gene expression; genetic library; genetic manipulation; genetic transcription; genetic translation; molecular biology; molecular cloning; mutant; natural gene amplification; nucleic acid sequence; oncogenes; oncoproteins; squamous cell carcinoma; tissue /cell culture

The promoter region of the EGF receptor gene contains SP1 binding sites which probably regulate its expression. A number of other possible regulatory sites have been detected in exonuclease protection assays. A full length cDNA clone of the EGF receptor gene has been constructed and used to make sense and antisense RNA. The sense RNA is translated into EGFR protein. Antisense RNA specifically inhibits this translation. Mutants of KB cells with low levels of EGF receptor have been isolated and are being characterized.

EGF受体基因的启动子区域包含SP1结合位点