MONOCLONAL ANTIBODIES TO CANCER CELLS

K.Chang已经开发出一种抗体(Kl)，可以与许多卵巢反应 癌症和间皮瘤。该抗原(CAK1)是一个40kD的糖基化抗原 通过PI锚定附着在细胞表面的蛋白质。为了获得基因 编码这种抗原，他已经筛选了一个lambda表达文库，使用 抗体，分离克隆，并对这些克隆进行测序。这种蛋白质 由这些cDNA编码的是一种与CAK1共享表位的胞浆蛋白 但很可能不是膜蛋白。然而，这种抗原是 在与CAK1相同的细胞中表达，并可能是同一成员 一家人。使用另一种方法，他最近获得了新的克隆 具有CAK1抗原膜形式的特征。 张博士还将单抗B3的反应性与 K1在人类肺癌中的作用，并已表明B3与许多 非小细胞肺癌，而K1不是。K1与 间皮瘤，而B3不是。因此，这两种抗体可以 可用于肺部肿瘤的鉴别诊断。

K. Chang has developed an antibody (Kl) that reacts with many ovarian cancers and mesotheliomas. The antigen (CAK1) is a 40 Kd glycosylated protein attached to the cell surface by a PI anchor. To obtain the gene encoding this antigen, he has screened a lambda expression library using antibodies, isolated clones and sequenced these clones. The protein encoded by these cDNAs is a cytosolic protein sharing an epitope with CAK1 but is probably not the membrane protein. Nevertheless, this antigen is expressed in the same cells as CAK1 and may be a member of the same family. Using an alternative approach, he has recently obtained new clones which have the characteristics of the membrane form of the CAK1 antigen. Dr. Chang has also compared the reactivity of monoclonal antibody B3 with that of K1 on human lung cancers and has shown that B3 reacts with many nonsmall cell carcinomas of the lung, whereas, K1 does not. K1 reacts with mesotheliomas, whereas, B3 does not. Therefore, these two antibodies can be used in the differential diagnosis of lung tumors.