EUKARYOTIC GENE REGULATION AND FUNCTION--THE METALLOTHIONEIN SYSTEM

真核基因调控与功能--金属硫蛋白系统

• 批准号: 3939265
• 负责人: D H HAMER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3939265
• 关键词: developmental genetics; eukaryote; gene expression; genetic manipulation; genetic mapping; genetic regulation; homeostasis; metalloproteins; metallothionein; molecular cloning; molecular genetics; nucleic acid sequence; yeasts

The metallothioneins provide a useful model to study how eukaryotic genes are regulated and how the gene products allow cellular adaptation and homeostasis. A mouse nuclear factor that binds to the metal control sequences of the mouse metallothionein-I gene has been identified and is being purified. Developmental regulation of the mammalian genes is being studied by transfection and transgenic animal experiments. Using yeast as a simple model system, trans-acting genes involved in metallothionein gene expression are being identified and cloned. Structure function studies of yeast metallothionein suggest the presence of preferred nucleation sites for the cooperative folding of this protein.

金属硫蛋白提供了一个有用的模型来研究如何 真核生物基因的调控以及基因产物如何 细胞适应和体内平衡。 小鼠核因子， 与小鼠的金属控制序列结合 金属硫蛋白-I基因已被鉴定并正在纯化。 哺乳动物基因的发育调节 通过转染和转基因动物实验进行研究。 使用 酵母作为一个简单的模型系统，反式作用基因参与 金属硫蛋白基因表达正在被鉴定和克隆。 酵母金属硫蛋白的结构和功能研究表明， 存在用于协同折叠的优选成核位点 这种蛋白质。