EUKARYOTIC GENE REGULATION AND FUNCTION--THE METALLOTHIONEIN SYSTEM

金属硫蛋白的调节和功能正在被分析 哺乳动物细胞和哺乳动物细胞中的分子遗传学和生化方法 酵母。一种与重金属对照相互作用的小鼠核蛋白 小鼠金属硫蛋白-I基因的序列已经被检测到，并且是 在体外受镉的调节。差异金属的结构基础 人金属硫蛋白-I亚型基因结合的研究 基因重排和转移实验。这些基因在体内的调控 开发和致癌基因的作用也在研究中。在酵母中，反式作用 改变金属硫蛋白基因调控的突变体已经被分离出来。一个 大量的突变体已经被工程改造到酵母中 金属硫蛋白编码序列，并被证明在确定 单个氨基酸在其结构和功能中的作用。

The regulation and function of metallothioneins are being analyzed by molecular genetic and biochemical approaches in both mammalian cells and yeast. A mouse nuclear protein that interacts with the heavy metal control sequences of the mouse metallothionein-I gene has been detected and is regulated by cadmium in vitro. The structural basis for differential metal binding of human metallothionein-I isoform genes has been investigated by gene shuffling and transfer experiments. Regulation of these genes during development and by oncogenes is also being studied. In yeast, trans-acting mutants that alter metallothionein gene regulation have been isolated. A substantial number of mutants have been engineered into the yeast metallothionein coding sequence and are proving useful in determining the role of individual amino acids in its structure and function.