EUKARYOTIC GENE REGULATION AND FUNCTION--THE METALLOTHIONEIN SYSTEM

真核基因调控与功能--金属硫蛋白系统

• 批准号: 3962979
• 负责人: D H HAMER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3962979
• 关键词: Menkes' syndrome; cadmium; developmental genetics; eukaryote; gene expression; genetic manipulation; genetic mapping; genetic regulation; genetic transcription; heavy metals; human tissue; hypocupremia; metalloproteins; metallothionein; molecular cloning; molecular genetics; nucleic acid sequence; oncogenes; oncogenic virus; radiotracer; regulatory gene; structural genes; tissue /cell culture; virus antigen; yeasts

The regulation and function of metallothioneins are being analyzed by molecular genetic and biochemical approaches in both mammalian cells and yeast. A mouse nuclear protein that interacts with the heavy metal control sequences of the mouse metallothionein-I gene has been detected and is regulated by cadmium in vitro. The structural basis for differential metal binding of human metallothionein-I isoform genes has been investigated by gene shuffling and transfer experiments. Regulation of these genes during development and by oncogenes is also being studied. In yeast, trans-acting mutants that alter metallothionein gene regulation have been isolated. A substantial number of mutants have been engineered into the yeast metallothionein coding sequence and are proving useful in determining the role of individual amino acids in its structure and function.

对金属硫蛋白的调控和功能进行了分析