EUKARYOTIC GENE REGULATION AND FUNCTION--THE METALLOTHIONEIN SYSTEM

真核基因调控与功能--金属硫蛋白系统

• 批准号: 4691799
• 负责人: D H HAMER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691799
• 关键词: Menkes' syndrome; copper; developmental genetics; eukaryote; gene expression; genetic manipulation; genetic mapping; genetic regulation; genetic transcription; heavy metals; hepatitis B virus group; human tissue; hypocupremia; metalloproteins; metallothionein; molecular cloning; nucleic acid sequence; oncogenes; oncogenic virus; radiotracer; regulatory gene; somatotropin; tissue /cell culture; virus antigen; yeasts

The regulation and function of metallothioneins are being analyzed by molecular genetic and biochemical approaches in both mammalian cells and yeast. Human metallothionein genes exhibit cell-specific expression that can be altered by the ras oncogene. The specificity of expression is correlated with differential metal-binding properties of the proteins. Attempts are in progress to detect and isolate the cellular factors involved in regulation by heavy metals and other agents. A copper-inducible metallothionein-like protein of yeast has been demonstrated to serve two functions: copper detoxification and negative autoregulation of its own expression. Both functions are complemented by mammalian metallothionein demonstrating the functional analogy between the higher and lower eukaryotic systems.

金属硫蛋白的调节和功能正在被分析 哺乳动物细胞和哺乳动物细胞中的分子遗传学和生化方法 酵母。人类金属硫蛋白基因表现出细胞特异性表达 可以被ras癌基因改变。表达的特异性是 与蛋白质不同的金属结合特性有关。 检测和分离细胞因子的尝试正在进行中 参与重金属和其他制剂的调节。一个 铜诱导酵母金属硫蛋白样蛋白的表达 证明有两种功能：铜解毒和负离子 对自身表达的自动调节。这两个功能都得到了以下补充 哺乳动物金属硫蛋白在功能上的类比 更高和更低的真核系统。