CELL-CELL INTERACTION BETWEEN ORAL ACTINOMYCETES AND OTHER ORAL BACTERIA

口腔放线菌和其他口腔细菌之间的细胞间相互作用

• 批准号: 3854183
• 负责人: P E KOLENBRANDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854183
• 关键词: Actinobacillus; Actinomycetales; Bacteroides gingivalis; Escherichia coli; Fusobacterium; Fusobacterium nucleatum; Streptococcus sanguis; adhesin; bacterial genetics; cell adhesion; cell cell interaction; genetic strain; membrane activity; membrane proteins; microorganism immunology; oral bacteria; tooth surface; transposon /insertion element

The focus of our research program is to understand the role of coaggregation in bacterial accretion of early colonizing bacteria on a clean tooth surface. The primary colonizers include actinomyces, streptococci, and veillonellae. The adhesion that mediates lactose- inhibitable coaggregation between Veillonella atypica PK1910 and human oral Streptococcus spp. has been identified as a 45 kDa-protein. It is selectively eluted by lactose from agarose-lactose beads as well as from cells of Streptococcus oralis 34, a veillonella coaggregation partner. Antisera that react with this veillonella protein also block specifically the lactose-inhibitable veillonella-streptococcus coaggregations, while lactose-noninhibitable coaggregations are unaffected. The 38 kDa-protein from S. gordonii PK488 appears to be an important surface molecule for early colonizing streptococci. All streptococci that express an immunologically cross-reactive protein, so far tested in our laboratory, coaggregate width A. naeslundii PK606. The gene encoding this protein in one of these streptococci, S. sanguis 12, has been cloned in E. coli and has been sequenced. The functionally equivalent gene from S. gordonii PK488 has been cloned in E. coli by constructing a lambda expression library of strain PK488 chromosomal DNA. This library was screened immunologically and a clone expressing the 38 kDA-protein was identified. While intergeneric coaggregation among oral bacteria is commonplace, intrageneric coaggregation among oral bacteria is highly unusual, except among streptococci. We have initiated studies aimed at developing a genetic system among the streptococci. We have shown that plasmids can be naturally transformed and transformed by electroporation, and we have shown that conjugative transposons can be transferred among oral streptococci.

我们研究计划的重点是了解 早期定植细菌在细菌生长过程中的共聚集 清洁牙齿表面。 主要定殖者包括放线菌、 链球菌和韦荣球菌。 介导乳糖的粘附 异型韦荣球菌 PK1910 和人类之间的可抑制共聚集 口腔链球菌属已被鉴定为 45 kDa 的蛋白质。 这是 乳糖选择性地从琼脂糖-乳糖珠以及 口腔链球菌 34 细胞，韦荣球菌共聚集伴侣。 与这种韦荣球菌蛋白发生反应的抗血清也能特异性阻断 乳糖抑制韦荣球菌-链球菌共聚集体，同时 乳糖不可抑制的共聚集不受影响。 来自 S. gordonii PK488 的 38 kDa 蛋白似乎是一个重要的 早期定植链球菌的表面分子。 所有链球菌 表达免疫交叉反应蛋白，迄今为止在我们的测试中进行了测试 实验室，共聚集体宽度 A. naeslundii PK606。 编码这个的基因 其中一种链球菌 S. sanguis 12 中的蛋白质已在大肠杆菌中克隆。 大肠杆菌并已测序。 来自 S 的功能等效基因。 gordonii PK488 已通过构建 lambda 在大肠杆菌中克隆 菌株PK488染色体DNA表达文库。 这个图书馆是 免疫学筛选并表达 38 kDA 蛋白的克隆 确定。 虽然口腔细菌之间的属间共聚集很常见， 口腔细菌之间的属内共聚集是非常不寻常的，除了 链球菌之中。 我们已经启动了旨在开发一种 链球菌之间的遗传系统。 我们已经证明质粒可以 自然转化和电穿孔转化，我们有 表明接合转座子可以在口腔之间转移 链球菌。