CELL-CELL INTERACTION BETWEEN ORAL ACTINOMYCETES AND OTHER ORAL BACTERIA

口腔放线菌和其他口腔细菌之间的细胞间相互作用

• 批准号: 3875196
• 负责人: P E KOLENBRANDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3875196
• 关键词: Actinomycetales; Bacteroides; Bacteroides gingivalis; Escherichia coli; Fusobacterium; Streptococcus salivarius; Streptococcus sanguis; adhesin; cell adhesion; cell cell interaction; dental plaque; genetic strain; gingiva; gram negative bacteria; gram positive bacteria; lectin; membrane activity; membrane proteins; microorganism immunology; oral bacteria; periodontium disorder

Coaggregation between S. gordonii PK488 and A. naeslundii PK606 is mediated by an adhesin of 38 kDa, which also may recognize salivary receptors. All streptococci that coaggregate with this actinomyces express an immunologically cross-reactive protein to this adhesin. From one of these streptococci, S. sanguis 12, the SsaB gene which encodes this adhesin has been cloned into E. coli and has been sequenced. It is 927 basepairs long, and encodes for a hydrophilic protein of molecular weight of 34,684 which has a hydrophobic signal peptide of 19 amino acids. Antisera against the cloned protein cross reacts with the 38 kDa protein from S. gordonii PK488. The ability of these streptococcal surface proteins to recognize both salivary receptors and actinomyces receptors provides the first example of a dual adhesive function of a surface protein recognizing both cellular and noncellular receptors. Coaggregation between V. atypica PK1910 and Streptococcus spp. appears to occur by two distinct adhesins, and one is galactoside-sensitive. Coaggregation-defective mutants that have lost the galactoside-sensitive coaggregation with streptococci also do not possess a protein of about 47 kDa. A protein of this size has been eluted from an N-acetylgalactosamine-agarose affinity column and is presumed to be the lactose-sensitive adhesin. While intergeneric coaggregation occurs with over 90% of the more than 700 human oral bacterial strains that we have examined, intrageneric coaggregation appears to be considerably more restricted. Only members of the genus Streptococcus and a few strains of Actinomyces exhibited intrageneric coaggregation. Both of these genera are initial colonizers of a clean tooth surface and intrageneric coaggregation may be of considerable importance in establishing the pioneer microbial community on a nascent surface. The results of each of these investigative approaches are focused on understanding the molecular basis of cell surface recognitions among oral bacteria and their role in microbial ecology.

戈登氏链霉菌PK488与内氏链霉菌PK606的共聚集 一种38 kDa的粘附素，它也可以识别唾液受体。全 与这种放线菌共同聚集的链球菌表达 这种粘附素的免疫交叉反应蛋白。从其中的一个 血链球菌12，编码这种粘附素的SSAB基因有 被克隆到大肠杆菌中，并进行了测序。它有927个基准线长， 编码一种相对分子质量为34,684的亲水蛋白 含有19个氨基酸的疏水信号肽。抗人血吸虫抗体 克隆的蛋白与戈登氏链霉菌PK488的38 kDa蛋白发生交叉反应。 这些链球菌表面蛋白识别两者的能力 唾液受体和放线菌受体提供了第一个例子 表面蛋白的双重黏附功能识别细胞和 非细胞受体。非典型性弧菌PK1910和pK1910的共聚集 链球菌属似乎是由两种不同的粘附素引起的，其中一种是 对半乳糖苷敏感。共聚集-缺失的突变体 与链球菌对半乳糖苷敏感的共聚集也不具有 约47 kDa的蛋白质。这种大小的蛋白质已经从一种 N-乙酰半乳糖胺-琼脂糖亲和层析柱，推测为 乳糖敏感的粘附素。而属间协同聚集发生在 在我们拥有的700多种人类口腔细菌菌株中，超过90%的 仔细检查，属内共聚集似乎要多得多 有限制。只有链球菌属的成员和少数几个菌株 放线菌具有属内共聚集性。这两个属都是 清洁牙齿表面的初始定植体和种内共聚集 可能对建立先锋微生物具有相当重要的意义 初现端倪的社区。每一项调查的结果 这些方法侧重于了解细胞表面的分子基础。 口腔细菌的认识及其在微生物生态学中的作用。