CELL-CELL INTERACTION BETWEEN ORAL ACTINOMYCETES AND OTHER ORAL BACTERIA

口腔放线菌和其他口腔细菌之间的细胞间相互作用

• 批准号: 4692620
• 负责人: P E KOLENBRANDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692620
• 关键词: Actinomycetales; Streptococcus mitis; Streptococcus sanguis; cell aggregation; cell cell interaction; gingiva; gram negative bacteria; microorganism immunology; oral bacteria; periodontium disorder

As additional information regarding cell-to-cell recognitions among oral bacteria is discovered, it is becoming increasing clear that a dynamic but organized microbial community exists in the oral cavity. The part of this complex community studied in this laboratory pertains especially to microbial ecology. Many of the bacteria that commonly inhabit this region were tested by an in vitro simulated plaque model. In the presence of potential competitors, several strains were tested as examples to support certain principles of coaggregation. Results of this study indicated that the oral cavity is replete with competitors and cohorts. Strains that belonged to different genera could recognize common third partners and prevented each other from coaggregating with them. Alternatively, demonstrations of multigeneric bacterial complexes were commonplace, indicating the similarity to associations of bacteria found in dental plaque. The surface structures that mediate these coaggregations were studied by using bacteriophage as surface probes and by isolating coaggregation-defective mutants. Identity of one receptor with a mediator of coaggregation was shown by altered coaggregation properties of bacteriophage-resistant mutants compared to their parent strains. To aid in further study of these surface structures, eight new actinomyces phage were isolated for the first time from human dental plaque. Studies with coaggregation-defective mutants revealed that certain outer membrane or surface proteins were altered or missing in the mutants but present in the parent strain. Results of a survey of the coaggregation properties of nearly 100 strains of Fusobacterium, Selenomonas, and Veillonella indicated that fusobacteria were very reactive, selenomonas hardly recognized any partners, and veillonella exhibited many lactose-inhibitable coaggregations. The results of each of these investigative approaches are focused on understanding the relationship of cell surface recognitions among oral bacteria and their role in microbial ecology.

作为关于口腔癌中细胞间分化的额外信息， 随着细菌的发现，越来越清楚的是，一个动态的，但 口腔中存在有组织的微生物群落。 这部分 本实验室研究的复杂群落尤其涉及 微生物生态学 许多通常栖息在这一地区的细菌 通过体外模拟菌斑模型进行测试。 存在下 潜在的竞争对手，几个菌株作为例子进行了测试，以支持 凝聚的某些原则。 这项研究的结果表明， 口腔中充满了竞争者和同伙。 的菌株 属于不同属的动物可以识别共同的第三伙伴， 阻止彼此聚合 可选择地， 多属细菌复合体的证明是常见的， 这表明与牙科中发现的细菌相关性相似， 斑块 介导这些共聚集的表面结构进行了研究， 使用噬菌体作为表面探针， 共聚集缺陷突变体。 一种受体与一种介质的同一性 共聚集性质的改变表明了共聚集的存在 噬菌体抗性突变体相比，其亲本菌株。 以帮助 在对这些表面结构进一步研究中， 首次从人牙菌斑中分离得到。 外贸 聚集缺陷突变体显示，某些外膜或 表面蛋白在突变体中改变或缺失，但在 亲株 近100种菌株共聚集特性的调查结果 对梭杆菌属、月形单胞菌属和韦荣氏球菌属的分析表明， 非常活跃，硒单胞菌几乎不认识任何伴侣， 韦荣氏球菌表现出许多乳糖可降解的聚集体。 结果 每一种调查方法的重点是了解 口腔细菌与其细胞表面识别的关系 在微生物生态学中的作用。